Burkholderia thailandensis: Genetic Manipulation

Current Protocols in Microbiology
Erin C Garcia

Abstract

Burkholderia thailandensis is a Gram-negative bacterium endemic to Southeast Asian and northern Australian soils. It is non-pathogenic; therefore, it is commonly used as a model organism for the related human pathogens Burkholderia mallei and Burkholderia pseudomallei. B. thailandensis is relatively easily genetically manipulated and a variety of robust genetic tools can be used in this organism. This unit describes protocols for conjugation, natural transformation, mini-Tn7 insertion, and allelic exchange in B. thailandensis. © 2017 by John Wiley & Sons, Inc.

References

Sep 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Matthew T G HoldenJulian Parkhill
May 24, 2005·Nature Methods·Kyoung-Hee ChoiHerbert P Schweizer
Dec 25, 2007·Applied and Environmental Microbiology·Kyoung-Hee ChoiHerbert P Schweizer
May 27, 2008·Applied and Environmental Microbiology·Ashley R BarrettTung T Hoang
Nov 29, 2008·BMC Genomics·Apichai TuanyokPaul Keim
Aug 4, 2009·Journal of Bacteriology·Josephine R ChandlerE Peter Greenberg
Aug 25, 2009·Applied and Environmental Microbiology·Carolina M LópezHerbert P Schweizer
Sep 21, 2010·Applied and Environmental Microbiology·Michael H NorrisTung T Hoang
Jul 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Christopher T FrenchJeff F Miller
Nov 7, 2013·MBio·Larry A GallagherColin Manoil
Jun 25, 2015·Microbiology Spectrum·Joseph E Peters

❮ Previous
Next ❯

Citations

Dec 14, 2018·The Journal of Biological Chemistry·Afsana SabrinAnne Grove

❮ Previous
Next ❯

Related Concepts

Related Papers

Current Protocols in Microbiology
Erin C Garcia, Peggy A Cotter
Environmental Science & Technology
Current Protocols in Microbiology
Anna C JacobsDaniel V Zurawski
Environmental Science & Technology
© 2022 Meta ULC. All rights reserved