Dec 19, 2012

Bursicon and neuropeptide cascades during the ecdysis program of the shore crab, Carcinus maenas

General and Comparative Endocrinology
S G WebsterJasmine H Sharp

Abstract

Very little is known regarding the release patterns of neuropeptides involved in ecdysis of crustaceans compared to insects. In particular, the dynamics of release of the insect cuticle hardening hormone bursicon, which has only recently been discovered in crustaceans, is unknown. Bursicon has not previously been identified as a circulating neurohormone in these animals. Since patterns of release were likely to be ephemeral, bursicon, as well as two other neurohormones involved in the ecdysis program in crustaceans, crustacean cardioactive peptide (CCAP) and crustacean hyperglycaemic hormone (CHH) were measured in single haemolymph samples in Carcinus maenas. For bursicon, an ultrasensitive time resolved-fluoroimmunoassay (TR-FIA) was developed, which firstly involved its characterisation by HPLC, bioassay and immunoassay. Simultaneous measurement of three neurohormones was performed at unparalleled levels of resolution, which has not previously been reported in any invertebrate. Additionally, expression patterns and architecture of neurones expressing both bursicon and CCAP were determined in the CNS during the moult cycle. Bursicon and CCAP are released in a massive surge, likely a single global exocytotic event on emergence,...Continue Reading

  • References27
  • Citations13

References

Mentioned in this Paper

Establishment and Maintenance of Localization
Inherited Factor II Deficiency
Molting
Calliphora vicina
Crabs
Nervousness
Imaginal Disc-derived Wing Expansion
Immunoreactivity
Class crustacea
Cavia

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