Bushen Tiaojing (II and III) Decoctions Activate MAPK14 and MAPK3/1 to Promote the Expression of Cumulus Expansion-Related Factors in Mice

Evidence-based Complementary and Alternative Medicine : ECAM
Xiao LiangYan-Cang Duan

Abstract

Bushen Tiaojing Decoctions (BSTJ-II-D and BSTJ-III-D) are used to assist pregnancy in clinical practice. In this study, we explored the ability of sequential administration of BSTJ-II-D and BSTJ-III-D to promote cumulus cell (CC) expansion and its underlying mechanisms in controlled ovarian hyperstimulation (COH) mice. Kunming mice were randomly divided into three groups. The normal group was injected intraperitoneally with saline, and distilled water was administered orally by gavage. As the COH model, mice were injected with GnRHa, eCG, and hCG. Subsequently, the BSTJD group received BSTJ-II-D and BSTJ-III-D orally by gavage, while the control group received distilled water. We evaluated CC expansion and oocyte first polar body (PB1) extrusion under a stereomicroscope. Serum levels of follicle-stimulating hormone (FSH) were detected by radioimmunoassay. The expression of the CC expansion-related factors PTX3 and PTGS2 was detected by immunofluorescence, western blot, and quantitative real-time-polymerase chain reaction analyses (qRT-PCR). Expression of p-MAPK14, p-MAPK3/1, MAPK14, and MAPK3/1 was detected by western blot analysis. Sequential administration of BSTJ-II-D and BSTJ-III-D promoted cumulus expansion and oocyte PB1 ...Continue Reading

References

May 1, 1993·Journal of Reproduction and Fertility·B C Vanderhyden
Aug 7, 2007·The Journal of Biological Chemistry·Laura ScarchilliAntonietta Salustri
Jan 29, 2008·The Journal of Biological Chemistry·Antonio InforzatoGiovanni Salvatori
Apr 7, 2010·The Journal of Clinical Investigation·Joanne S Richards, Stephanie A Pangas
Sep 6, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·R ProchazkaL Nemcova
Jan 23, 2014·Reproductive Biomedicine Online·Waleed F MareiAli A Fouladi-Nashta
Oct 17, 2014·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Dan-Dan CuiMing-Min Zhang
May 20, 2015·Developmental Biology·Satoshi SugimuraRobert B Gilchrist
May 11, 2016·Theriogenology·Darryl L RussellJeremy G Thompson
Jan 19, 2018·International Journal of Molecular Sciences·Eva Nagyova
Feb 7, 2018·Matrix Biology : Journal of the International Society for Matrix Biology·Antonietta SalustriAntonella Camaioni
Jul 17, 2018·Endocrinology·Rebecca L RobkerDarryl L Russell

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electrophoresis

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