Busulfan administration produces toxic effects on epididymal morphology and inhibits the expression of ZO-1 and vimentin in the mouse epididymis

Bioscience Reports
Fang FangChengliang Xiong

Abstract

Busulfan is an alkane sulphonate currently used as an anticancer drug and to prepare azoospermic animal models, because it selectively destroys differentiated spermatogonia in the testes. However, few studies have focussed on the exact effects of busulfan treatment on the epididymis currently. The present study assessed the effect of busulfan on epididymal morphology and the blood-epididymis barrier in mice. We treated mice with a single injection of busulfan and detected the effect at different time points. We showed that busulfan was toxic to the morphological structure and function of the epididymis. Furthermore, busulfan treatment down-regulated the epididymal expression of vimentin and zonula occludens-1 (ZO-1) at the mRNA and protein levels. In addition, there was an increase in total androgen receptor (AR) levels, whereas the estrogen receptor-α (ER-α) levels were reduced, both in the caput and cauda regions after busulfan treatment, which may be secondary to the testicular damage. In conclusion, our study describes the effects of busulfan administration on the mouse epididymis and also provides a potential understanding of male infertility arising from chemotherapy-related defects in the epididymis.

References

Apr 1, 1991·Molecular and Cellular Endocrinology·A KrongradM J McPhaul
Nov 25, 1967·Nature·M C Orgebin-Crist
Feb 1, 1995·Biology of Reproduction·B Robaire, R S Viger
Feb 2, 2002·Endocrine·A C Pereyra-MartinezJ A Resko
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Julie DufresneDaniel G Cyr
Feb 15, 2003·Endocrinology·Nadine Ezer, Bernard Robaire
Apr 4, 2003·Biology of Reproduction·Clayton J BrinsterKyle E Orwig
May 16, 2003·Reproductive Biology and Endocrinology : RB&E·Serge CarreauSonia Bourguiba
May 30, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·T T TurnerQ A T Nguyen
Jul 3, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Cleida A OliveiraRex A Hess
Feb 15, 2007·International Journal of Andrology·K PospechovaV Semecky
Sep 22, 2007·Molecular Reproduction and Development·Carlos Reyes-MorenoRobert Sullivan
Apr 9, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·P J O'ShaughnessyP J Baker
Jan 13, 2009·Human Reproduction Update·Gail A Cornwall
Apr 18, 2009·Maturitas·Jin Li, Farook Al-Azzawi
Jan 26, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Melissa G MendezRobert D Goldman
Feb 4, 2010·Arquivos brasileiros de endocrinologia e metabologia·Marilia T C C PatrãoMaria Christina W Avellar
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Serge Carreau, Rex A Hess
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Serge CarreauIsabelle Galeraud-Denis
Jul 24, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Elizabeth M SnyderBarry T Hinton
Oct 12, 2010·Biology of Reproduction·Avenel JosephRex A Hess
Jan 7, 2011·Biology of Reproduction·Payal MitalJannette M Dufour
Mar 29, 2011·Journal of Andrology·Winnie W C ShumSylvie Breton
Mar 29, 2011·Journal of Andrology·Rex A HessCatarina S Porto
Jun 4, 2011·Cellular and Molecular Life Sciences : CMLS·Arun Satelli, Shulin Li
Jul 19, 2011·Journal of Andrology·Bernard Robaire, Mahsa Hamzeh
Mar 20, 2012·Cell and Tissue Research·Clémence BelleannéeRobert Sullivan
Nov 20, 2012·Expert Opinion on Drug Metabolism & Toxicology·Ariane Galaup, Angelo Paci
Feb 12, 2013·Advances in Experimental Medicine and Biology·Evemie Dubé, Daniel G Cyr
Jan 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Li-Sophie Z RathjeAnnica K B Gad

❮ Previous
Next ❯

Citations

Aug 28, 2019·Cytotechnology·Begum Yurdakok-DikmenAyhan Filazi
Aug 4, 2020·The Journal of Toxicological Sciences·Lu YuanXiujun Zhang
Aug 21, 2020·Andrologia·Salah ElbashirAshok Agarwal
Aug 8, 2020·Drug and Chemical Toxicology·Sunny O AbarikwuChigozie L Onuah
Mar 14, 2021·Tissue Engineering and Regenerative Medicine·Heba F IbrahimAmira Y Medwar

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Protein Assay

Software Mentioned

SPSS
IPP
Metrical

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.