Butyl benzyl phthalate suppresses the ATP-induced cell proliferation in human osteosarcoma HOS cells

Toxicology and Applied Pharmacology
Pei-Shan Liu, Chih-Ying Chen

Abstract

Butyl benzyl phthalate (BBP), an endocrine disruptor present in the environment, exerts its genomic effects via intracellular steroid receptors and elicits non-genomic effects by interfering with membrane ion-channel receptors. We previously found that BBP blocks the calcium signaling coupled with P2X receptors in PC12 cells (Liu & Chen, 2006). Osteoblast P2X receptors were recently reported to play a role in cell proliferation and bone remodeling. In this present study, the effects of BBP on ATP-induced responses were investigated in human osteosarcoma HOS cells. These receptors mRNA had been detected, named P2X4, P2X7, P2Y2, P2Y4, P2Y5, P2Y9, and P2Y11, in human osteosarcoma HOS cells by RT-PCR. The enhancement of cell proliferation and the decrease of cytoviability had both been shown to be coupled to stimulation via different concentrations of ATP. BBP suppressed the ATP-induced calcium influx (mainly coupled with P2X) and cell proliferation but not the ATP-induced intracellular calcium release (mainly coupled with P2Y) and cytotoxicity in human osteosarcoma HOS cells. Suramin, a common P2 receptor's antagonist, blocked the ATP-induced calcium signaling, cell proliferation, and cytotoxicity. We suggest that P2X is mainly re...Continue Reading

References

Jul 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M P RathboneR F Del Maestro
Feb 1, 1971·Cancer·R M McAllisterB H Landing
Apr 1, 1995·Environmental Health Perspectives·S H Safe
Mar 1, 1996·Cell Biology International·P A WackymL Hoffman
Feb 1, 1996·Calcified Tissue International·S Shimegi
Jan 27, 1999·Biochemical and Biophysical Research Communications·M NakaiY Shimohigashi
Jul 27, 2000·American Journal of Physiology. Cell Physiology·E NakamuraF Izumi
Oct 18, 2000·Bone·A HoebertzT R Arnett
May 9, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A GartlandW B Bowler
May 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A HoebertzT R Arnett
Mar 23, 2002·European Journal of Morphology·G MenghiM M Hurley
Jun 26, 2003·Trends in Pharmacological Sciences·Astrid HoebertzGeoffrey Burnstock
Nov 18, 2004·American Journal of Surgery·Stanley ZaslauDavid W McFadden
Dec 8, 2004·Biochemical and Biophysical Research Communications·Hiroyuki IharaAkifumi Togari
Dec 28, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Damian C GenetosRandall L Duncan
May 11, 2005·Biochemical and Biophysical Research Communications·Milena RomanelloPaola D'Andrea
Nov 11, 2005·APMIS. Supplementum·Niklas Rye Jørgensen
Dec 20, 2005·Toxicology and Applied Pharmacology·Pei-Shan Liu, Yi-Yin Chen
Jan 19, 2006·American Journal of Physiology. Renal Physiology·Yun Jung Lee, Ho Jae Han
Jan 19, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Bronwen A J EvansJack Ham
Apr 18, 2006·Bone·Isabel R OrrissTimothy R Arnett
Dec 7, 2006·Archives of Pharmacal Research·Mi-Jung YoonDong-Ku Kim
Feb 16, 2007·Journal of Cellular Physiology·Damian C GenetosHenry J Donahue
Sep 6, 2008·Journal of Cellular Biochemistry·Jonathan D Kaunitz, Dean T Yamaguchi
Feb 19, 2009·Purinergic Signalling·Matthew W GrolS Jeffrey Dixon
Feb 20, 2009·British Journal of Pharmacology·S M AlqallafE J Kidd
Mar 31, 2009·Journal of Cellular Biochemistry·M G SabbietiL Marchetti
Apr 25, 2009·Journal of Applied Toxicology : JAT·Anne-Marie SaillenfaitJean-Philippe Sabaté

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Citations

Apr 13, 2013·Indian Journal of Occupational and Environmental Medicine·Vijaya Prakash Krishnan MuthaiahJayaraman Gopalsamy
Sep 13, 2012·Journal of Osteoporosis·Elena AdinolfiAnna Lisa Giuliani
Jun 26, 2013·Purinergic Signalling·Geoffrey Burnstock, Francesco Di Virgilio
Aug 15, 2013·Purinergic Signalling·Geoffrey BurnstockIsabel R Orriss
Sep 19, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Firdous Ahmad BhatN Srinivasan
May 18, 2016·Development, Growth & Differentiation·Alessandro CutarelliUmberto Tarantino
Jan 13, 2016·Journal of Cellular Physiology·J B Noronha-Matos, P Correia-de-Sá
Sep 17, 2014·PloS One·Anna Lisa GiulianiElena Adinolfi
Jun 6, 2020·Purinergic Signalling·Marzia CarluccioRenata Ciccarelli
Jun 26, 2020·Frontiers in Pharmacology·Romain LaraShaun McNulty
Oct 11, 2017·Frontiers in Pharmacology·Geoffrey Burnstock
Apr 16, 2021·Purinergic Signalling·Maria EllegaardNiklas Rye Jørgensen

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