Butyrate and the cytokine-induced alpha1-proteinase inhibitor release in intestinal epithelial cells

European Journal of Clinical Investigation
D FaustJ Stein

Abstract

Alpha1-proteinase inhibitor (alpha1-PI), an anti-inflammatory protein thought to play a role in the intestinal inflammation, is synthesised by and released from the intestinal epithelial cells. IL-1beta is a key proinflammatory cytokine in the abnormal immune response that occurs in inflammatory bowel disease. Butyrate is a normal luminal constituent in the colon, known to be of benefit in preventing inflammatory bowel disease. Direct modes of action of butyrate in intestinal inflammation have been poorly studied so far. The aim of this study was to investigate the effects of butyrate on cytokine-mediated alpha1-PI release in intestinal epithelial cells. Differentiated Caco-2 cells were incubated with IL-1beta in the presence or absence of 2 mM butyrate. Alpha1-PI expression in the cells was evaluated by Western blot analysis and alpha1-PI release by ELISA. Treatment with butyrate alone had no effect on alpha1-PI expression in differentiated Caco-2 cells. However, treatment of the cells with 2 mM butyrate significantly reduced the alpha1-PI level in IL-1beta-treated cells. In the cell culture medium, the presence of butyrate impaired the IL-1beta-induced alpha1-PI release to 17-35%. The treatment induced no change in the number...Continue Reading

References

May 1, 1994·Clinical Science·T D Wardle, L A Turnberg
Jul 1, 1994·The Journal of Surgical Research·W FrankelJ Rombeau
Oct 1, 1993·The Journal of Clinical Investigation·E P MolmentiD C Rubin
Apr 1, 1996·Digestive Diseases and Sciences·O C VelázquezJ L Rombeau
May 1, 1996·Gut·W E Roediger, S Millard
Feb 1, 1997·European Journal of Gastroenterology & Hepatology·W ScheppachH Kasper
Feb 14, 1998·Journal of Cellular Physiology·M J EngleD H Alpers
Feb 19, 2000·Biochemical and Biophysical Research Communications·A WächtershäuserJ Stein
Mar 29, 2000·Advances in Experimental Medicine and Biology·G D'Argenio, G Mazzacca
Apr 25, 2000·Annual Review of Medicine·K A Papadakis, S R Targan
Jun 2, 2000·Biochemical and Biophysical Research Communications·A WächtershäuserJ Stein
Feb 22, 2001·Biochemical and Biophysical Research Communications·A Wächtershäuser, J Stein
Apr 21, 2001·Gastroenterology·J A Pilla, S D Zucker

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Citations

May 13, 2003·The Proceedings of the Nutrition Society·Elizabeth A WilliamsJohn C Mathers
Mar 3, 2012·Journal of Pediatric Gastroenterology and Nutrition·Maciej ChichlowskiDavid A Mills
Mar 4, 2011·Respiratory Medicine·Sabina M JanciauskieneTobias Welte
Sep 30, 2004·International Journal of Cancer. Journal International Du Cancer·Daniela DiehlStefan Bauersachs

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