Butyrate Enhances Desensitization Induced by Oral Immunotherapy in Cow's Milk Allergic Mice

Mediators of Inflammation
Marlotte M VonkBetty C A M van Esch

Abstract

In previous studies, we showed that a fructo-oligosaccharide- (FOS-) supplemented diet enhanced oral immunotherapy (OIT) efficacy in a mouse model for cow's milk allergy. Fermentation of FOS by intestinal bacteria leads to production of short-chain fatty acids (SCFA) including butyrate. To investigate the contribution of butyrate in the enhanced efficacy of OIT + FOS. C3H/HeOuJ mice were sensitized and received OIT with or without FOS or butyrate supplementation. After treatment, whole blood was collected to conduct a basophil activation test (BAT) and allergen challenges were performed to measure acute allergic symptoms. CD4 + CD25 + regulatory T cells (Tregs) were isolated from treated mice or differentiated in vitro and used in a bone marrow-derived mast cell (BMMC) suppression assay. Cecum content was collected to analyze SCFA concentrations. Allergen-induced basophil activation was reduced in OIT + butyrate samples compared to OIT. Accordingly, the acute allergic skin response and mast cell degranulation upon challenge were reduced in OIT + butyrate and OIT + FOS mice compared to sensitized controls. Butyrate was increased in the cecum content of OIT + FOS mice compared to OIT mice and sensitized controls. Treg-mediated BM...Continue Reading

References

Jun 15, 1995·The New England Journal of Medicine·G D SherN F Olivieri
Jul 21, 2007·Nature Protocols·Massimo C FantiniChristoph Becker
Jan 13, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M N TorreroE Mitre
Sep 25, 2009·The Journal of Biological Chemistry·Toshiro NikiMitsuomi Hirashima
May 7, 2010·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Thomas EiweggerZsolt Szépfalusi
Mar 8, 2011·The Journal of Allergy and Clinical Immunology·Nicholas J OsborneUNKNOWN HealthNuts Investigators
Aug 6, 2011·European Journal of Pharmacology·Sander de KivitLinette E M Willemsen
Jun 16, 2012·The Journal of Allergy and Clinical Immunology·Natalija NovakCezmi Akdis
Dec 18, 2013·Brain, Behavior, and Immunity·Caroline G M de TheijeRaish Oozeer
Jan 7, 2014·Nature Medicine·Aurélien TrompetteBenjamin J Marsland
Jan 17, 2015·The Journal of Allergy and Clinical Immunology·Mimi L K TangSusan Donath
Apr 8, 2015·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·A Wesley BurksBryan M Harvey
Jul 5, 2015·Current Allergy and Asthma Reports·David Chiang, M Cecilia Berin
Oct 7, 2015·The Journal of Allergy and Clinical Immunology. in Practice·Cara BeckUNKNOWN HealthNuts Investigators
Oct 10, 2015·The British Journal of Nutrition·Cheng Jun JinIna Bergheim
Nov 26, 2015·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Marta Vazquez-Ortiz, Paul J Turner
Nov 26, 2015·The Journal of Nutritional Biochemistry·Hanying ZhangMei-Jun Zhu
Jul 28, 2016·Journal of Gastroenterology·Mingming SunYingzi Cong
Apr 13, 2017·Nutrition Reviews·Christina M van der BeekKaatje Lenaerts
Jun 29, 2017·Immunological Reviews·Craig McKenzieCharles R Mackay
Sep 4, 2017·Seminars in Immunology·Deborah M Hussey FreelandKari C Nadeau
Oct 13, 2017·Clinical and Translational Allergy·Marlotte M VonkJohan Garssen
Nov 21, 2017·The Journal of Allergy and Clinical Immunology. in Practice·Evelien M VermeulenUNKNOWN HealthNuts investigators
Nov 22, 2017·The Journal of Allergy and Clinical Immunology·Scott H Sicherer, Hugh A Sampson
Dec 6, 2017·Allergy·G B PajnoUNKNOWN EAACI Allergen Immunotherapy Guidelines Group

❮ Previous
Next ❯

Citations

May 30, 2020·Children·Christina L NanceAikaterini Anagnostou
Jul 30, 2020·International Journal of Molecular Sciences·Margherita Di CostanzoGiacomo Biasucci
Jun 3, 2021·Nutrients·Domenico GarganoPaola Iovino
Jun 29, 2021·Frontiers in Immunology·Benjamin Zepeda-OrtegaJohn O Warner

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
ELISA
IEC

Software Mentioned

GraphPad Prism
GraphPad

Related Concepts

Related Feeds

Basophils

Basophils are myeloid cells with a high affinity IgE receptor and is involved in inflammatory responses during allergy. Discover the latest research on Basophils here.