Buyang Huanwu Decoction (BYHWD) Enhances Angiogenic Effect of Mesenchymal Stem Cell by Upregulating VEGF Expression After Focal Cerebral Ischemia

Journal of Molecular Neuroscience : MN
Junhong YangDi Zhang

Abstract

Buyang Huanwu decoction (BYHWD) has been used for centuries to treat paralysis and stroke. Previously, we have demonstrated that BYHWD combined with mesenchymal stem cell (MSC) transplantation attenuates ischemic injury partly by upregulating angiogenesis. However, the mechanisms of this drug for stroke treatment are not completely understood. Here, we aimed to clarify the mechanism of BYHWD on angiogenesis mediated by MSCs. Firstly, we verified microvessels with a size of 50-100 nm produced by either MSCs or MSCs treated by 500 μg/ml BYHWD. These exosomes were purified and found to be able to activate vascular endothelial growth factor (VEGF) expression in endothelial cells (ECs). Moreover, exosomes from MSCs and MSCs treated by BYHWD induced elevated microRNA (miRNA)-126 expression and reduced miR-221 and miR-222 expression. In MSCs, disruption of dicer, an enzyme responsible for miRNA maturation, by dicer small interfering RNA (siRNA), or RNase pretreatment abolished this ability of the exosomes. Additionally, exosomes from MSCs treated by BYHWD promoted VEGF and Ki-67 expression and augmented vascular density in rat brain after bilateral carotid artery ligation. In conclusion, our study revealed that BYHWD exposure augmente...Continue Reading

References

Aug 3, 2002·Nature Reviews. Immunology·Clotilde ThérySebastian Amigorena
Jul 20, 2006·Blood·Laura PolisenoGiuseppe Rainaldi
Mar 4, 2008·Clinical and Investigative Medicine. Médecine Clinique Et Experimentale·WeiDe ZhongQiShan Dai
May 15, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Chien-Yuan LinTeng-Nan Lin
Aug 13, 2008·Developmental Cell·Shusheng WangEric N Olson
Aug 13, 2008·Developmental Cell·Jason E FishDeepak Srivastava
Apr 25, 2009·Journal of the American Society of Nephrology : JASN·Stefania BrunoGiovanni Camussi
May 19, 2009·Current Opinion in Genetics & Development·Shusheng Wang, Eric N Olson
Feb 9, 2010·Stem Cell Research·Ruenn Chai LaiSai Kiang Lim
Jun 21, 2011·Seminars in Immunopathology·Douglas D Taylor, Cicek Gercel-Taylor
Jun 5, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Marco RagusaMichele Purrello
Jun 26, 2012·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Masahito KawaboriNagara Tamaki
Dec 4, 2012·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Michiyuki MiyamotoNagara Tamaki
Oct 29, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Tamas KriskaWilliam B Campbell

❮ Previous
Next ❯

Citations

Feb 20, 2016·Molecular Neurobiology·Zhiping HuLiuwang Zeng
Dec 23, 2015·Neural Regeneration Research·Anuradha Kalani, Neetu Tyagi
May 24, 2016·Scientific Reports·Ping ZhangChuanlu Jiang
Jun 9, 2016·Journal of Clinical Medicine·Sai-Wang SetoHosen Kiat
Sep 24, 2016·BMC Complementary and Alternative Medicine·Jiangquan LiaoJie Wang
Oct 14, 2016·Journal of Stroke·Oh Young BangGyeong Joon Moon
Mar 7, 2019·American Journal of Physiology. Cell Physiology·Qingyi MaWilliam J Pearce
Apr 18, 2018·Molecular Neurobiology·Nicole OsierJessica Gill
May 16, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yi ZhangWei-Juan Gao
Dec 20, 2018·Frontiers in Immunology·Joana R FerreiraRaquel M Gonçalves
Jun 11, 2019·Oxidative Medicine and Cellular Longevity·Masoud AlasvandIlaria Peluso
Apr 5, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Lin LuoBaiyan Liu
Oct 8, 2020·Stem Cells Translational Medicine·Vanessa CastelliAnnamaria Cimini
May 14, 2021·Translational Stroke Research·Yiyang LiYonghua Zhao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Related Papers

Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine
Tie-Binq QuLi-Sheng Chu
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine
Ying-Hong TangBei-Yang Chen
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine
Li TongJian-gang Shen
© 2021 Meta ULC. All rights reserved