B,Z conformations and mechanism of the Z-B-coil transitions of the self-complementary deoxy-hexanucleotide d(C-G-m5C-G-C-G) by 1H-NMR and CD spectroscopy

Journal of Biomolecular Structure & Dynamics
J A CavaillèsS Tran-Dinh

Abstract

The helical structures of d(C-G-m5C-G-C-G) were studied in aqueous solution at various salt concentrations and temperatures by CD and 1H-NMR spectroscopy. At room temperature only the B form is observed in 0.1 M NaCl whereas the B and Z forms are simultaneously present in 1.8 M NaCl. At high salt concentration (4 M NaCl) the Z form is largely predominant (greater than 95%). The Z form proton resonances were assigned by using the polarisation transfer method (between B and Z at 1.8 M NaCl) and by proton-proton decoupling (at high salt concentration). The Z-B-Coil transitions were studied as a function of temperature with the 1.8 M NaCl solution. At high temperature (95 degrees C) only the coil form (S) is present. Below 55 degrees C the coil proportion is negligible, and the B-Z exchange is slow. The disappearance of the coil gives rise at first to the B form and on lowering the temperature the Z proportion increases to the detriment of the B form. Proton linewidth, relaxation and polarisation transfer studies confirm the conclusion in the previous report on d(m5C-G-C-G-m5C-G) (Tran-Dinh et al Biochemistry 1984 in the press) that Z exchanges only with B whereas the latter also exchanges with S,Z in equilibrium B in equilibrium S...Continue Reading

References

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Citations

Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J B Chaires, J M Sturtevant
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A DelbarreB P Roques
Feb 11, 1986·Nucleic Acids Research·P W DavisT Neilson
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Sep 1, 1989·Biopolymers·G A Thomas, W L Peticolas
Jun 1, 1985·Journal of Biomolecular Structure & Dynamics·J A TabouryJ Igolen

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