c-Cbl facilitates fibronectin matrix production by v-Abl-transformed NIH3T3 cells via activation of small GTPases

Oncogene
A M TeckchandaniAlexander Y Tsygankov

Abstract

The protooncogenic protein c-Cbl has been shown to act as a multivalent adaptor and a negative regulator of protein tyrosine kinase-mediated signaling. Recent studies have implicated it in the regulation of cell adhesion-related events. We have previously shown that c-Cbl facilitates adhesion and spreading of v-Abl-transformed fibroblasts, and that these effects are dependent on its tyrosine phosphorylation. However, the mechanisms mediating effects of c-Cbl on fibroblast adhesion remain poorly understood. In this study we demonstrate that the tyrosine phosphorylation-dependent effect of c-Cbl on adhesion of v-Abl-transformed fibroblasts is primarily mediated by an increase in fibronectin matrix deposition by these cells. This increase in fibronectin matrix deposition and, hence, in cell adhesion is dependent on cytoskeletal rearrangements induced by RhoA, Rac1 and, possibly, Rap1 activation caused by c-Cbl. The observed activation of these GTPases is mediated by the recruitment of phosphatidylinositol-3' kinase, CrkL and Vav2 to the C-terminal tyrosine residues of c-Cbl. The results of this study also demonstrate that ubiquitination is essential for the observed effects of c-Cbl on fibronectin matrix production and cell adhesion.

References

Jun 20, 1978·Annals of the New York Academy of Sciences·A VaheriJ Wartiovaara
Oct 1, 1977·The Journal of Small Animal Practice·J W Northington, M M Juliana
Jan 1, 1992·Cold Spring Harbor Symposia on Quantitative Biology·A J Ridley, A Hall
Jan 1, 1983·Experimental Cell Biology·R RajaramanC A Murdock
Jun 1, 1995·Current Biology : CB·P J Parker
Jan 1, 1995·Annals of Human Genetics·E P HenskeD J Kwiatkowski
Dec 1, 1995·Molecular and Cellular Biology·M TanakaB J Mayer
Aug 1, 1995·Biochemical Society Transactions·C D Nobes, A Hall
May 1, 1996·The Biochemical Journal·G M BokochA E Traynor-Kaplan
Jun 1, 1996·The Journal of Cell Biology·M Chrzanowska-Wodnicka, K Burridge
Sep 13, 1996·The Journal of Biological Chemistry·S KleinD B Rifkin
Oct 18, 1996·The Journal of Biological Chemistry·G M BokochU G Knaus
Jul 6, 1996·British Dental Journal·P Varley
Oct 25, 1996·The Journal of Biological Chemistry·A Y TsygankovJ B Bolen
Sep 1, 1996·The British Journal of Dermatology·N HunzelmannT Krieg
Jan 1, 1996·Annual Review of Cell and Developmental Biology·E Ruoslahti
Feb 1, 1997·Current Opinion in Cell Biology·N Tapon, A Hall
Jan 15, 1997·The EMBO Journal·B FrankeJ L Bos
May 30, 1997·The Journal of Biological Chemistry·T JascurT Mustelin
Jul 25, 1997·The Journal of Biological Chemistry·T GotohS Hattori

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Citations

Jul 23, 2014·Immunology and Cell Biology·Tiffanny N NewmanAlexander Y Tsygankov
Jun 8, 2013·Journal of Cellular Physiology·Hojin Lee, Alexander Y Tsygankov
Apr 12, 2008·The International Journal of Biochemistry & Cell Biology·Hojin LeeAlexander Y Tsygankov
Jun 3, 2006·Journal of Cellular Physiology·Gayathri Swaminathan, Alexander Y Tsygankov
Aug 19, 2010·Journal of Cellular Biochemistry·Hojin Lee, Alexander Y Tsygankov
Jun 1, 2005·Experimental Cell Research·Anjali M TeckchandaniAlexander Y Tsygankov
May 10, 2005·Experimental Cell Research·Anjali M TeckchandaniAlexander Y Tsygankov
Apr 27, 2004·Oncogene·Elena A FeshchenkoAlexander Y Tsygankov
Oct 19, 2001·Oncogene·A Y TsygankovG Swaminathan

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