c-di-GMP Regulates Various Phenotypes and Insecticidal Activity of Gram-Positive Bacillus thuringiensis

Frontiers in Microbiology
Yang FuJin He

Abstract

C-di-GMP has been well investigated to play significant roles in the physiology of many Gram-negative bacteria. However, its effect on Gram-positive bacteria is less known. In order to more understand the c-di-GMP functions in Gram-positive bacteria, we have carried out a detailed study on the c-di-GMP-metabolizing enzymes and their physiological functions in Bacillus thuringiensis, a Gram-positive entomopathogenic bacterium that has been applied as an insecticide successfully. We performed a systematic study on the ten putative c-di-GMP-synthesizing enzyme diguanylate cyclases (DGCs) and c-di-GMP-degrading enzyme phosphodiesterases (PDEs) in B. thuringiensis BMB171, and artificially elevated the intracellular c-di-GMP level in BMB171 by deleting one or more pde genes. We found increasing level of intracellular c-di-GMP exhibits similar activities as those in Gram-negative bacteria, including altered activities in cell motility, biofilm formation, and cell-cell aggregation. Unexpectedly, we additionally found a novel function exhibited by the increasing level of c-di-GMP to promote the insecticidal activity of this bacterium against Helicoverpa armigera. Through whole-genome transcriptome profile analyses, we found that 4.3% of...Continue Reading

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Citations

Dec 21, 2019·Environmental Microbiology·Cao ZhengJin He
May 31, 2020·FEMS Microbiology Reviews·Wen YinJin He
Mar 19, 2019·Microbial Biotechnology·Paulina JacekStanisław Bielecki
Dec 29, 2020·Critical Reviews in Microbiology·Wen YinJin He
Jun 19, 2021·Frontiers in Microbiology·Lu LiuJin He
Jul 25, 2021·International Journal of Molecular Sciences·Marko NedeljkovićEric John Sundberg
Jul 30, 2021·Journal of Medicinal Chemistry·Teng-Fei XuanJing Lin
Jun 8, 2021·Journal of Bacteriology·Timothy M HermanasGeorge C Stewart

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Methods Mentioned

BETA
PCR
electrophoresis
RNA-seq
reverse transcription PCR

Software Mentioned

KOBAS
Origin
R
FASTX
KOBAS Orthology Based Annotation System )
Bowtie2
DEGseq package
Primer
BLAST

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