Abstract
Neurons in Caenorhabditis elegans and other nematodes have been thought to lack classical action potentials. Unexpectedly, we observe membrane potential spikes with defining characteristics of action potentials in C. elegans AWA olfactory neurons recorded under current-clamp conditions. Ion substitution experiments, mutant analysis, pharmacology, and modeling indicate that AWA fires calcium spikes, which are initiated by EGL-19 voltage-gated CaV1 calcium channels and terminated by SHK-1 Shaker-type potassium channels. AWA action potentials result in characteristic signals in calcium imaging experiments. These calcium signals are also observed when intact animals are exposed to odors, suggesting that natural odor stimuli induce AWA spiking. The stimuli that elicit action potentials match AWA's specialized function in climbing odor gradients. Our results provide evidence that C. elegans neurons can encode information through regenerative all-or-none action potentials, expand the computational repertoire of its nervous system, and inform future modeling of its neural coding and network dynamics.
Citations
Jul 12, 2019·ELife·Connor Brennan, Alexander Proekt
Jul 2, 2019·PloS One·Martina NicolettiSimonetta Filippi
May 27, 2020·Anesthesiology·Gregory S WirakChristopher W Connor
Mar 7, 2019·Scientific Reports·Tomomi ShindouIchiro N Maruyama
Aug 23, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Samantha Hughes, Tansu Celikel
Nov 14, 2019·ELife·May DobosiewiczCornelia I Bargmann
Jun 20, 2020·Scientific Reports·Petrus Van der AuweraIsabel Beets
Dec 4, 2020·International Journal of Neural Systems·Loïs NaudinThibaut Démare
Mar 28, 2019·Cell Reports·Angela R WildMark L Dell'Acqua
Jan 25, 2021·Chemosphere·Huanliang LiuDayong Wang
Jan 8, 2021·Cell and Tissue Research·Sandra C KochVolker Hartenstein
Apr 16, 2020·Journal of Neurophysiology·Christina K JohnsonLaura Bianchi
Mar 7, 2021·Biomolecules·Elizabeth S Li, Margaret S Saha
Mar 1, 2021·Pharmacology Research & Perspectives·Sebastián GiuntiMaría José De Rosa
Mar 12, 2021·Genetics·Denise M FerkeyNoelle D L'Etoile
Mar 27, 2021·Frontiers in Systems Neuroscience·Vivek Kurien GeorgeGabriel A Silva
Mar 23, 2021·Frontiers in Neuroscience·Beck StrohmerLeon Bonde Larsen
May 20, 2021·Physical Review. E·M Rahimi-MajdM N Najafi
Jun 22, 2021·Genetics·Scott W EmmonsManuel Zimmer
May 27, 2021·Genetics·Bhavya RaviKevin M Collins
Nov 6, 2021·ELife·Aylesse Sordillo, Cornelia I Bargmann
Nov 10, 2021·PLoS Computational Biology·Javier J HowSreekanth H Chalasani
Dec 12, 2021·The European Journal of Neuroscience·Bennet G SakelarisEleni Gourgou