C. elegans Blastomeres Clear the Corpse of the Second Polar Body by LC3-Associated Phagocytosis

Cell Reports
Gholamreza FazeliAnn M Wehman

Abstract

To understand how undifferentiated pluripotent cells cope with cell corpses, we examined the clearance of polar bodies born during female meiosis. We found that polar bodies lose membrane integrity and expose phosphatidylserine in Caenorhabditis elegans. Polar body signaling recruits engulfment receptors to the plasma membrane of embryonic blastomeres using the PI3K VPS-34, RAB-5 GTPase and the sorting nexin SNX-6. The second polar body is then phagocytosed using receptor-mediated engulfment pathways dependent on the Rac1 ortholog CED-10 but undergoes non-apoptotic programmed cell death independent of engulfment. RAB-7 GTPase is required for lysosome recruitment to the polar body phagosome, while LC3 lipidation is required for degradation of the corpse membrane after lysosome fusion. The polar body phagolysosome vesiculates in an mTOR- and ARL-8-dependent manner, which assists its timely degradation. Thus, we established a genetic model to study clearance by LC3-associated phagocytosis and reveal insights into the mechanisms of phagosome maturation and degradation.

Citations

Sep 19, 2019·Médecine sciences : M/S·Mathilde GalaisMartine Biard-Piechaczyk
Aug 7, 2020·The FEBS Journal·Roni Levin-Konigsberg, Adriana R Mantegazza
May 8, 2020·Cell Discovery·Sascha Martens, Dorotea Fracchiolla
Aug 15, 2020·Frontiers in Cell and Developmental Biology·Gholamreza FazeliAnn M Wehman
Aug 14, 2019·Genetics·Jeremy Nance, Christian Frøkjær-Jensen
Apr 11, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Oliver Florey, Michael Overholtzer
May 6, 2021·Cell Discovery·Sascha Martens, Dorotea Fracchiolla
Jul 7, 2021·Human Reproduction Update·Meng-Meng Shan, Shao-Chen Sun

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.