C. elegans nucleostemin is required for larval growth and germline stem cell division.

PLoS Genetics
Michelle M Kudron, V Reinke

Abstract

The nucleolus has shown to be integral for many processes related to cell growth and proliferation. Stem cells in particular are likely to depend upon nucleolus-based processes to remain in a proliferative state. A highly conserved nucleolar factor named nucleostemin is proposed to be a critical link between nucleolar function and stem-cell-specific processes. Currently, it is unclear whether nucleostemin modulates proliferation by affecting ribosome biogenesis or by another nucleolus-based activity that is specific to stem cells and/or highly proliferating cells. Here, we investigate nucleostemin (nst-1) in the nematode C. elegans, which enables us to examine nst-1 function during both proliferation and differentiation in vivo. Like mammalian nucleostemin, the NST-1 protein is localized to the nucleolus and the nucleoplasm; however, its expression is found in both differentiated and proliferating cells. Global loss of C. elegans nucleostemin (nst-1) leads to a larval arrest phenotype due to a growth defect in the soma, while loss of nst-1 specifically in the germ line causes germline stem cells to undergo a cell cycle arrest. nst-1 mutants exhibit reduced levels of rRNAs, suggesting defects in ribosome biogenesis. However, NST...Continue Reading

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Citations

May 12, 2012·Journal of Biomedicine & Biotechnology·Li-Wei LeeSzecheng J Lo
Dec 25, 2008·The Journal of Biological Chemistry·Liudmila RomanovaNobuaki Kikyo
Sep 19, 2009·PLoS Genetics·Laura E FuhrmanAlejandro Aballay
Apr 21, 2010·PloS One·Gayla HadwigerMichael L Nonet
Feb 24, 2012·Molecular Biology of the Cell·Xiaomin WangZonglie Hong
Jul 11, 2012·Stem Cells and Development·Haruhiko ShugoAtsushi Hirao
Nov 19, 2015·Developmental Biology·Debasmita RoyE Jane Albert Hubbard
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Jul 7, 2012·PloS One·Darina KorčekováChristian Lanctôt
Feb 18, 2015·PloS One·Tasha K MendesPeter R Boag
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Nov 28, 2013·G3 : Genes - Genomes - Genetics·Anita G FernandezFabio Piano
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Methods Mentioned

BETA
GTPase
GTPases
PCR
transgenic
pull-down
electrophoresis
in

Software Mentioned

AlphaEaseFC

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