C. elegans rrf-1 mutations maintain RNAi efficiency in the soma in addition to the germline.

PloS One
Caroline Kumsta, Malene Hansen

Abstract

Gene inactivation through RNA interference (RNAi) has proven to be a valuable tool for studying gene function in C. elegans. When combined with tissue-specific gene inactivation methods, RNAi has the potential to shed light on the function of a gene in distinct tissues. In this study we characterized C. elegans rrf-1 mutants to determine their ability to process RNAi in various tissues. These mutants have been widely used in RNAi studies to assess the function of genes specifically in the C. elegans germline. Upon closer analysis, we found that two rrf-1 mutants carrying different loss-of-function alleles were capable of processing RNAi targeting several somatically expressed genes. Specifically, we observed that the intestine was able to process RNAi triggers efficiently, whereas cells in the hypodermis showed partial susceptibility to RNAi in rrf-1 mutants. Other somatic tissues in rrf-1 mutants, such as the muscles and the somatic gonad, appeared resistant to RNAi. In addition to these observations, we found that the rrf-1(pk1417) mutation induced the expression of several transgenic arrays, including the FOXO transcription factor DAF-16. Unexpectedly, rrf-1(pk1417) mutants showed increased endogenous expression of the DAF-1...Continue Reading

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Citations

Feb 26, 2014·Proceedings of the National Academy of Sciences of the United States of America·Aaron M KershnerJudith Kimble
Apr 15, 2014·Methods : a Companion to Methods in Enzymology·Francis Raj Gandhi AmritArjumand Ghazi
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Oct 24, 2014·PLoS Genetics·Mor Levi-FerberSivan Henis-Korenblit
Jun 27, 2017·Developmental Biology·Lara M LindenDavid R Sherwood
May 17, 2018·PLoS Genetics·Margarita R HernandezGyörgyi Csankovszki
Jun 8, 2018·PLoS Biology·Yanmei ZhaoKerry Kornfeld
Mar 7, 2019·Nucleic Acids Research·Evandro A De-SouzaMarcelo A Mori
Jan 2, 2015·G3 : Genes - Genomes - Genetics·Yunting Chen, Iva Greenwald
Feb 23, 2019·Scientific Reports·Lina ZouDi Chen
Sep 12, 2018·International Journal of Molecular Sciences·Livia UlicnaPavel Hozak

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Methods Mentioned

BETA
transgenic
fluorescence microscopy
nuclear translocation
PCR
electrophoresis

Software Mentioned

GraphPad Prism
Twitcher
Stata
ImageJ

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