C. elegans sym-1 is a downstream target of the hunchback-like-1 developmental timing transcription factor.

Cell Cycle
Ryusuke NiwaFrank J Slack

Abstract

In the nematode Caenorhabditis elegans, the let-7 microRNA (miRNA) and its family members control the timing of key developmental events in part by directly regulating expression of hunchback-like-1 (hbl-1). C. elegans hbl-1 mutants display multiple developmental timing deficiencies, including cell cycle defects during larval development. While hbl-1 is predicted to encode a transcriptional regulator, downstream targets of HBL-1 have not been fully elucidated. Here we report using microarray analysis to uncover genes downstream of HBL-1. We established a transgenic strain that overexpresses hbl-1 under the control of a heat shock promoter. Heat shock-induced hbl-1 overexpression led to retarded hypodermal structures at the adult stage, opposite to the effect seen in loss of function (lf) hbl-1 mutants. The microarray screen identified numerous potential genes that are upregulated or downregulated by HBL-1, including sym-1, which encodes a leucine-rich repeat protein with a signal sequence. We found an increase in sym-1 transcription in the heat shock-induced hbl-1 overexpression strain, while loss of hbl-1 function caused a decrease in sym-1 expression levels. Furthermore, we found that sym-1(lf) modified the hypodermal abnorma...Continue Reading

Citations

Aug 16, 2011·Journal of Visualized Experiments : JoVE·Kassandra L GuthmuellerAndrea M Holgado
Mar 17, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Tamar D ResnickAnn E Rougvie
Jul 21, 2020·Molecular Systems Biology·Milou Wm MeeuseHelge Großhans

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