PMID: 8605096Mar 1, 1996Paper

c-erbB-2 in astrocytomas: infrequent overexpression by immunohistochemistry and absence of gene amplification by fluorescence in situ hybridization

British Journal of Cancer
H HaapasaloJ Isola

Abstract

Recent studies suggest that aberrations of c-erbB-2 may be involved in astrocytic brain tumours. We screened immunohistochemically c-erbB2 protein (p185) expression in 94 astrocytic grade 1-4 neoplasms of the brain. The amplification of the c-erbB-2 oncogene was investigated in protein overexpression cases by dual colour fluorescence in situ hybridisation (FISH). p185 overexpression was correlated with p53 and epidermal growth factor receptor (EGFR) expression, as well as with clinicopathological features. Only two anaplastic (grade 3) astrocytomas and one glioblastoma (grade 4) showed overexpression of p185 protein by immunohistochemistry (monoclonal MAb1 antibody TA250), whereas none of the grade 1-2 astrocytomas was positive. Interestingly, the expression of p185 was confined solely to the cytoplasm of neoplastic astrocytic cells and not to the cell membranes as found in malignancies with amplification of the c-erbB-2 oncogene. Two of the three overexpression cases were also positive by EGFR. No amplification of the c-erbB-2 gene was observed by FISH in the three tumours with immunohistochemical p185 overexpression or seven weakly positive/negative tumours. In conclusion, our results suggest that p185 overexpression is infre...Continue Reading

Citations

May 15, 2003·Brain Pathology·Hannu HaapasaloYlermi Soini
Jun 16, 2007·Journal of Neuro-oncology·Jean-François MineoPhong Dam Hieu
Apr 22, 2015·Oncogene·C Y ChuaW Zhang
Nov 15, 2008·Cancer Immunology, Immunotherapy : CII·J F M JacobsP M Hoogerbrugge
Mar 25, 2010·Diagnostic Pathology·Sasha GulatiSverre H Torp
Aug 2, 2011·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Joanna ReszećZenon Mariak
Mar 13, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Saik MuallaogluOzgur Ozyilkan
Jul 12, 2013·International Journal of Surgical Pathology·Ingunn Syversen WaageSverre Helge Torp
Aug 7, 2003·American Journal of Clinical Oncology·Vijay KokaRalph Levitt
Jun 1, 2007·Applied Immunohistochemistry & Molecular Morphology : AIMM·Denise M HaynikRichard A Prayson
Aug 13, 2005·Advances in Anatomic Pathology·Christine E Fuller, Arie Perry

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