c-fos reduces growth factor requirements for mitogenic stimulation of L6 rat myoblasts

Journal of Cellular Physiology
M Rahm, T Sejersen

Abstract

Addition of fetal calf serum (FCS) to serum-deprived L6J1 rat myoblasts increases fos-like immunoreactivity. The nuclear immunoreactivity reached a maximum 2 h after serum addition. Effects of the c-fos protein on myoblast proliferation were analyzed in L6J1 rat myoblasts transfected with the murine c-fos gene under control of a metallothionein promoter. L6J1 myoblasts with elevated expression of transfected c-fos reached higher cell densities than neo transfected control myoblasts when approaching a stationary phase in normal culture conditions (5% FCS). The differences in cell densities were even more pronounced at low serum concentrations (0.5% FCS). c-fos transfected cells also had a faster growth rate than did control cells in serum-free medium supplemented with calcium chloride, lithium chloride, sodium selenite, hydrocortisone, and insulin. The cell morphology of c-fos transfected L6J1 myoblasts was not affected compared to control myoblasts. These results suggest that c-fos protein expression in L6J1 myoblasts is activated by serum and that mitogenic stimulation of L6J1 myoblasts is facilitated by the presence of elevated amounts of c-fos protein.

References

Apr 1, 1988·Molecular and Cellular Biology·K T RiabowolJ R Feramisco
May 1, 1989·Journal of Cellular Physiology·M RahmT Sejersen
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F MeijlinkI M Verma
Nov 20, 1987·Cell·I M Verma, P Sassone-Corsi
May 20, 1988·Science·F J RauscherB R Franza
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J T HoltA W Nienhuis
Feb 1, 1987·Molecular and Cellular Biology·K Nishikura, J M Murray
May 1, 1987·Experimental Cell Research·M P LeibovitchJ Harel
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·D Yaffe

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Citations

Dec 31, 1990·Biochemical and Biophysical Research Communications·M Rahm, A Hultgårdh-Nilsson
Jun 1, 1990·Journal of Cellular Physiology·M RahmN R Ringertz

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