c-Jun activation is required for 4-hydroxytamoxifen-induced cell death in breast cancer cells

Oncogene
A MadeoAnna Maria Musti

Abstract

The c-Jun N-terminal kinase (JNK) has been shown to mediate tamoxifen-induced apoptosis in breast cancer cells. However, the downstream mediators of the JNK pathway linking tamoxifen to effectors of apoptosis have yet to be identified. In this study, we analysed whether c-Jun, the major nuclear target of JNK, has a role in tamoxifen-induced apoptosis of SkBr3 breast cancer cells. We show that before DNA fragmentation and caspase 3/7 activation, cytotoxic concentrations of 4-hydroxytamoxifen (OHT) induced JNK-dependent phosphorylation of c-Jun at JNK sites earlier shown to regulate c-Jun-mediated apoptosis. In addition, OHT induced ERK-dependent expression of c-Fos and transactivation of an AP-1-responsive promoter. In particular, the ectopic expression of dominant-negative constructs blocking either AP-1 activity or c-Jun N-terminal phosphorylation prevented DNA fragmentation after OHT treatment. Furthermore, both c-Fos expression and c-Jun N-terminal phosphorylation preceded OHT-dependent activation of caspase 3-7 in different types of tamoxifen-sensitive cancer cells, but not in OHT-resistant LNCaP prostate cancer cells. Taken together, our results indicate that the c-Jun/c-Fos AP-1 complex has a pro-apoptotic role in OHT-tre...Continue Reading

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Citations

Apr 23, 2014·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Sae-lo-oom LeeJie-Young Song
Jan 28, 2012·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hai-Ying ChenLe-Xin Wang
Nov 28, 2013·The International Journal of Biochemistry & Cell Biology·Marco PupoAnna Maria Musti
Aug 17, 2016·International Journal of Cancer. Journal International Du Cancer·Jianling XiaWangjun Liao

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