c-Jun binds the N terminus of human TAF(II)250 to derepress RNA polymerase II transcription in vitro

The Journal of Biological Chemistry
Tricia N LivelyJ A Goodrich

Abstract

c-Jun is an oncoprotein that activates transcription of many genes involved in cell growth and proliferation. We studied the mechanism of transcriptional activation by human c-Jun in a human RNA polymerase II transcription system composed of highly purified recombinant and native transcription factors. Transcriptional activation by c-Jun depends on the TATA-binding protein (TBP)-associated factor (TAF) subunits of transcription factor IID (TFIID). Protein-protein interaction assays revealed that c-Jun binds with high specificity to the largest subunit of human TFIID, TAF(II)250. The region of TAF(II)250 bound by c-Jun lies in the N-terminal 163 amino acids. This same region of TAF(II)250 binds to TBP and represses its interaction with TATA boxes, thereby decreasing DNA binding by TFIID. We hypothesized that c-Jun is capable of derepressing the effect of the TAF(II)250 N terminus on TFIID-driven transcription. In support of this hypothesis, we found that c-Jun increased levels of TFIID-driven transcription in vitro when added at high concentrations to a DNA template lacking activator protein 1 (AP-1) sites. Moreover, c-Jun blocked the repression of TBP DNA binding caused by the N terminus of TAF(II)250. In addition to revealing ...Continue Reading

References

Nov 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L Weis, D Reinberg
Sep 1, 1992·Genes & Development·J A SutherlandT Kouzarides
Oct 1, 1990·Molecular and Cellular Biology·C AbateT Curran
Mar 2, 1989·Nature·J W Lillie, M R Green
Jun 9, 1995·The Journal of Biological Chemistry·R A ColemanB F Pugh
Jun 9, 1995·The Journal of Biological Chemistry·R A Coleman, B F Pugh
Sep 1, 1995·Current Biology : CB·M F Carey
Sep 12, 1996·Nature·S S WalkerM R Green
Sep 1, 1996·Trends in Biochemical Sciences·C P Verrijzer, R Tjian
Nov 1, 1996·Genes & Development·G OrphanidesD Reinberg
Jan 7, 1997·Proceedings of the National Academy of Sciences of the United States of America·J NishikawaY Nakatani
Apr 1, 1997·Current Opinion in Cell Biology·M KarinE Zandi
Feb 3, 1998·Molecular and Cellular Biology·T KokuboY Nakatani
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·J F Kugel, J A Goodrich
Oct 6, 1998·Annual Review of Biochemistry·J L Workman, R E Kingston
Jun 6, 2000·Trends in Biochemical Sciences·S Malik, R G Roeder
Jun 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·T KotaniT Kokubo

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Citations

Jan 25, 2006·Nature Methods·Tuan N Nguyen, James A Goodrich
Dec 24, 2009·Nature Reviews. Cancer·Pablo Lopez-BergamiZe'ev Ronai
Aug 10, 2004·Nature Structural & Molecular Biology·Celso A EspinozaJames A Goodrich
Apr 3, 2004·Molecular and Cellular Biology·Shinya TakahataTetsuro Kokubo
Mar 22, 2014·Frontiers in Oncology·Jennifer R RibeiroRichard N Freiman
Aug 10, 2004·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Koji KasaharaTetsuro Kokubo
Apr 19, 2017·Molecular and Cellular Biology·R A ColemanW L Liu
May 29, 2003·Oncogene·Thorsten Bartusel, Karl-Heinz Klempnauer
Apr 5, 2003·The Journal of Biological Chemistry·Christine MunzAlexander Pintzas
Oct 18, 2019·ACS Medicinal Chemistry Letters·David RemillardNathanael S Gray

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