c-Jun N-terminal kinase-mediated stabilization of microsomal prostaglandin E2 synthase-1 mRNA regulates delayed microsomal prostaglandin E2 synthase-1 expression and prostaglandin E2 biosynthesis by cardiomyocytes

The Journal of Biological Chemistry
Norbert DegouseeBarry Rubin

Abstract

Microsomal prostaglandin (PG) E(2) synthase-1 (mPGES-1) catalyzes the terminal step in the biosynthesis of PGE(2), a key proinflammatory mediator. The purpose of this study was to elucidate the regulation of mPGES-1 mRNA expression in cardiomyocytes, define the role of JNK enzymes in this process, and characterize the role of mPGES-1 in cardiomyocyte PGE(2) biosynthesis. In neonatal cardiomyocytes, interleukin-1beta and lipopolysaccharide (LPS) both stimulated mPGES-1 mRNA expression and increased mPGES-1 mRNA stability and protein synthesis but failed to increase mPGES-1 mRNA transcription. Treatment with the JNK1/2 inhibitor, SP600125, abrogated the increases in mPGES-1 mRNA stability, mPGES-1 protein synthesis, and PGE(2) release induced by interleukin-1beta or LPS. mPGES-1 protein synthesis was observed in LPS-stimulated neonatal cardiomyocytes from jnk1(-/-) or jnk2(-/-) mice. In contrast, infection of jnk1(-/-) cardiomyocytes with an adenovirus encoding phosphorylation-resistant JNK2 (ad-JNK2-DN), or of jnk2(-/-) cardiomyocytes with ad-JNK1-DN, significantly decreased LPS-stimulated mPGES-1 protein synthesis. Similarly, co-infection with ad-JNK1-DN and ad-JNK2-DN attenuated LPS-stimulated mPGES-1 protein synthesis in card...Continue Reading

References

Aug 13, 1993·Cell·A B Sachs
Nov 1, 1995·Trends in Biochemical Sciences·C Y Chen, A B Shyu
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·P J JakobssonB Samuelsson
Aug 14, 1999·Biochimica Et Biophysica Acta·K WatanabeT Suzuki
Jun 22, 2001·The Journal of Biological Chemistry·N UenoI Kudo
Feb 28, 2002·Biochemical and Biophysical Research Communications·Naomi TanikawaKikuko Watanabe
Mar 8, 2002·Hypertension·Mariela Mendez, Margot C LaPointe
Jun 12, 2002·The Journal of Biological Chemistry·Geum-Yi KimEileen Friedman
Jan 22, 2003·The Biochemical Journal·Jenny BainPhilip Cohen
Jul 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Catherine E TrebinoLaurent P Audoly
Nov 20, 2003·The Journal of Biological Chemistry·Dania Alarcon-Vargas, Ze'ev Ronai
Aug 11, 2004·The Journal of Biological Chemistry·Wen Hong TohKanaga Sabapathy
Feb 22, 2005·The Journal of Biological Chemistry·Catherine E TrebinoLaurent P Audoly

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Citations

Mar 23, 2011·American Journal of Respiratory Cell and Molecular Biology·Paul D Bozyk, Bethany B Moore
Jul 29, 2006·Arthritis Research & Therapy·Marjolaine GossetClaire Jacques
Oct 22, 2008·Journal of Lipid Research·Noelle B VargasGerald M Wilson
May 31, 2007·Proceedings of the National Academy of Sciences of the United States of America·Annika O HofstetterEric Herlenius
Mar 12, 2009·Physiology & Behavior·Emilie PecchiJean-Denis Troadec
Jul 21, 2007·Journal of Hepatology·Laurent MagneMichèle Garlatti
Aug 19, 2007·Liver International : Official Journal of the International Association for the Study of the Liver·Yasushi TakiiKiyoshi Migita
Jun 18, 2009·Expert Opinion on Therapeutic Targets·Jitesh P IyerAbhijit Ray
Apr 29, 2009·Molecular and Cellular Biochemistry·Hana RadilovaMartin Buncek
Sep 20, 2007·Pharmacological Reviews·Bengt SamuelssonPer-Johan Jakobsson
Apr 17, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Matthew D MartensJoseph W Gordon

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