c-KIT messenger RNA and protein expression and mutations in canine cutaneous mast cell tumors: correlations with post-surgical prognosis

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Mery GiantinMauro Dacasto

Abstract

Cutaneous mast cell tumors (MCTs) are among the most common neoplasms in dogs and show a highly variable biologic behavior. Histological grading, cell proliferation markers, and KIT immunohistochemistry are typically used to predict post-surgical prognosis. In the present study, c-KIT messenger RNA (mRNA) expression was measured in canine MCTs and its relationship with tumor grade, immunohistochemical staining pattern, post-surgical prognosis, and mutations was investigated. A significant increase of c-KIT mRNA was observed in MCTs versus healthy skin and surgical margins. Mutations were observed in 8.3% of cases. The KIT staining pattern was investigated for both grading systems. In particular, staining pattern III was associated with grade II (G2) and G3 MCTs, while staining patterns I and II were associated with G1 and G2 MCTs. Considering the 2-tier histological grading, the high grade was mainly associated with pattern III (71%) while the low grade was associated with patterns II (70%) and I (28%). A weak association between the KIT staining pattern and outcome was also observed. The results obtained suggest that c-KIT mRNA is overexpressed in canine MCT, although the fold variations were not associated with the protein lo...Continue Reading

References

Sep 1, 1984·Veterinary Pathology·A K PatnaikE G MacEwen
Nov 1, 1994·Veterinary Pathology·J P SimoesM Butine
Jan 8, 1999·The Journal of Veterinary Medical Science·K KuboY Takamori
Apr 21, 1999·Experimental Hematology·C A LondonE N Geissler
Dec 3, 1999·The International Journal of Biochemistry & Cell Biology·D Linnekin
Feb 12, 2000·Journal of Leukocyte Biology·M BoissanM Arock
Feb 14, 2002·Trends in Molecular Medicine·Esther ZwickAxel Ullrich
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 21, 2002·American Journal of Veterinary Research·Sue DowningCheryl A London
Jul 11, 2003·The Veterinary Clinics of North America. Small Animal Practice·Cheryl A London, Bernard Seguin
Jan 23, 2004·Journal of the American Veterinary Medical Association·Amelia M SimpsonAmiya K Patnaik
Mar 9, 2004·Journal of Comparative Pathology·R PreziosiM Paltrinieri
Apr 1, 2004·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Cameron L R JonesCheryl A London
Apr 22, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Maria MoriniLuciana Mandrioli
Jul 30, 2005·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·M KiupelJ B Kaneene
Sep 1, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Federica RivaLauretta Turin
Jan 24, 2006·Journal of the American Veterinary Medical Association·Ryan P FulcherAmiya K Patnaik
Apr 4, 2006·BMC Cancer·Joshua D WebsterVilma Yuzbasiyan-Gurkan
Apr 14, 2006·Neoplasia : an International Journal for Oncology Research·Joshua D WebsterMatti Kiupel
Aug 23, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Lauretta TurinFederica Riva
May 11, 2007·Veterinary Pathology·E M RomansikC A London
Jun 26, 2007·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Hans-Peter HornyPeter Valent
Jul 9, 2008·Immunopharmacology and Immunotoxicology·Letizia PassantinoAntonella Perillo
Jul 23, 2008·Molecular Cancer Research : MCR·Sébastien LetardPatrice Dubreuil
Aug 9, 2008·Veterinary Immunology and Immunopathology·Keitaro OhmoriKenichi Masuda
Nov 5, 2008·Veterinary Dermatology·Monika M WelleSilvia Rüfenacht
May 21, 2009·Veterinary and Comparative Oncology·E L KrickK U Sorenmo
Sep 8, 2009·Topics in Companion Animal Medicine·Cheryl A London
Nov 17, 2010·Veterinary Pathology·J J ThompsonR A Foster

❮ Previous
Next ❯

Citations

Nov 1, 2013·Journal of the American Veterinary Medical Association·Kathryn M PratschkeCatherine G Lamm
Aug 13, 2013·BioMed Research International·Gennaro AltamuraGiuseppe Borzacchiello
Jun 10, 2016·Veterinary and Comparative Oncology·S SabattiniG Bettini
Nov 4, 2015·The Veterinary Journal·R KlopfleischA D Gruber
Feb 12, 2014·Veterinary Pathology·S SabattiniG Bettini
Apr 29, 2015·Journal of Feline Medicine and Surgery·Silvia SabattiniGiuliano Bettini
Jan 9, 2014·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Anna GranatoFranco Mutinelli
Oct 5, 2018·Journal of Applied Genetics·Miluse VozdovaJiri Rubes
Jan 31, 2020·Veterinary and Comparative Oncology·Miluse VozdovaJiri Rubes
Oct 28, 2019·Veterinary Medicine and Science·Vanessa S TamlinAnne E Peaston
Oct 2, 2019·Veterinary and Comparative Oncology·Miluse VozdovaJiri Rubes
Jun 20, 2018·Frontiers in Veterinary Science·Galina V IlyinskayaPeter M Chumakov
Sep 15, 2020·Veterinary and Comparative Oncology·Byung-Joon SeungJung-Hyang Sur
Mar 17, 2021·Veterinary and Comparative Oncology·Jennifer Ostrand FreytagBruno Cogliati

❮ Previous
Next ❯

Methods Mentioned

BETA
phosphotransferase
genetic modifications
biopsies
electrophoresis
Assay
PCR

Software Mentioned

ChromasPro
Ensembl Genome Browser
UPL Assay Design Centre
Primer3

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.