C-methylflavonoids isolated from Callistemon lanceolatus protect PC12 cells against Abeta-induced toxicity

Planta medica
So-Young ParkDongho Lee

Abstract

Increased beta-amyloid (Abeta) production and its aggregation to the oligomeric state is considered to be a major cause of Alzheimer's disease (AD). Therefore, reducing Abeta-induced neurotoxicity could provide a suitable means of prevention or intervention in the disease course of AD. The neuroprotective effects of isolates from Callistemon lanceolatus DC. (Myrtaceae) against Abeta were evaluated using PC12 cells. To evaluate the effects of Abeta on apoptotic cell death and the effects of Bcl-2 family proteins and caspase-3, TUNEL assays and Western blotting were performed, respectively. Substantial fractionation and purification of the EtOAc-soluble extract of the aerial parts of C. lanceolatus afforded six flavonoids, 4',5-dihydroxy-6,8-dimethyl-7-methoxyflavanone (1), eucalyptin (2), 8-demethyleucalyptin (3), sideroxylin (4), syzalterin (5), and quercetin (6). Compounds 1, 5, and 6 were found to protect PC12 cells effectively against Abeta-induced toxicity. In particular, compound 1 showed the most promising neuroprotective effect with an ED (50) value of 6.7 microM in terms of decreasing Abeta-induced apoptotic cell death, and this was accompanied by a decrease in caspase-3 activation and an increase in Bcl-2/Bax ratio. Th...Continue Reading

Citations

Nov 6, 2010·Archives of Pharmacal Research·So-Young Park
Sep 13, 2012·Natural Product Research·A A GoharW S Aboelmaaty
Jun 19, 2015·Journal of Natural Products·Dan Tian, John R Porter
Oct 6, 2010·The Journal of Pathology·Pablo Blanco Martínez de MorentinMiguel López
Aug 14, 2018·Current Drug Targets·Duraiswamy BasavanMohan K S Kumar
Aug 10, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mengfei YuanWencai Ye
Jun 10, 2020·Molecular Biology Reports·Fenxiang LiuYu Zhang

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