C-peptide signals via Galpha i to protect against TNF-alpha-mediated apoptosis of opossum kidney proximal tubular cells

Journal of the American Society of Nephrology : JASN
Nawal M Al-RasheedNigel J Brunskill

Abstract

Cell loss by apoptosis occurs in renal injury such as diabetic nephropathy. TNF-alpha is a cytokine that induces apoptosis and has been implicated in the pathogenesis of diabetic nephropathy. The aim was to investigate whether C-peptide or insulin could modulate TNF-alpha-mediated cell death in opossum kidney proximal tubular cells and to examine the mechanism(s) of any effects observed. C-peptide and insulin protect against TNF-alpha-induced proximal tubular cell toxicity and apoptosis. Cell viability was analyzed by methylthiazoletetrazolium assay; cell viability was reduced to 60.8 +/- 2.7% of control after stimulation with 300 ng/ml TNF-alpha. Compromised cell viability was reversed by pretreatment with 5 nM C-peptide or 100 nM insulin. TNF-alpha-induced apoptosis was detected by DNA nick-end labeling and by measuring histone associated DNA fragments using ELISA. By ELISA assay, 300 ng/ml TNF-alpha increased apoptosis by 145.8 +/- 4.9% compared with controls, whereas 5 nM C-peptide and 100 nM insulin reduced apoptosis to 81.6 +/- 4.8 and 77.4 +/- 3.1% of control, respectively. The protective effects of C-peptide and insulin were associated with activation of NF-kappaB. Activation of NF-kappaB by C-peptide was pertussis toxi...Continue Reading

References

Nov 1, 1996·Science·D J Van AntwerpI M Verma
Dec 24, 1997·Journal of the American Society of Nephrology : JASN·A OrtizJ Egido
Feb 28, 1998·The Journal of Biological Chemistry·F BertrandG Cherqui
Jul 9, 1998·The New England Journal of Medicine·P FiorettoM Mauer
Sep 12, 1998·Kidney International·M SjöquistB L Johansson
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·V J PalombellaM B Grisham
Sep 15, 1999·Nature·J A Romashkova, S S Makarov
Apr 26, 2000·American Journal of Physiology. Endocrinology and Metabolism·J WahrenH Jörnvall
Apr 28, 2000·Diabetic Medicine : a Journal of the British Diabetic Association·B L JohanssonJ Wahren
Feb 13, 2001·The International Journal of Biochemistry & Cell Biology·H Wajant, P Scheurich
Sep 29, 2001·Kidney International·B SamnegårdM Sjöquist
Feb 28, 2002·Naunyn-Schmiedeberg's Archives of Pharmacology·Dan-Yang HuangVolker Vallon
Jun 18, 2002·Journal of Cellular and Molecular Medicine·A OrtizJ Egido
Sep 12, 2002·Cellular and Molecular Life Sciences : CMLS·J ShafqatH Jörnvall
Dec 13, 2002·Current Drug Targets. Immune, Endocrine and Metabolic Disorders·A OrtizJ Egido
Mar 26, 2003·Cell Death and Differentiation·H WajantP Scheurich
Sep 3, 2003·Diabetes/metabolism Research and Reviews·Zhen-Guo LiAnders A F Sima
Jan 30, 2004·Journal of the American Society of Nephrology : JASN·Nigel J Brunskill
Nov 19, 2004·Cellular and Molecular Life Sciences : CMLS·Z ZhongA V Chibalin

❮ Previous
Next ❯

Citations

Jan 20, 2007·Diabetologia·J WahrenH Jörnvall
Jan 17, 2007·Diabetologia·L LuziA Caumo
Jun 17, 2009·Journal of Cardiovascular Pharmacology·Pitchai BalakumarMadhu B Anand-Srivastava
Jan 26, 2011·Shock·Ranjit S ChimaBasilia Zingarelli
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Nermin A H SadikOlfat G Shaker
Aug 27, 2009·Biologics : Targets & Therapy·Lina NordquistBradley T Andresen
Jan 30, 2014·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Michal UsarekJadwiga Bryla
Sep 23, 2014·Cardiovascular Research·Mahendra Prasad BhattKwon-Soo Ha
Jun 2, 2011·International Journal of Hypertension·Shoko HoritaToshiro Fujita
Jul 29, 2006·Diabetes/metabolism Research and Reviews·Marco BuglianiPiero Marchetti
May 28, 2011·Diabetes/metabolism Research and Reviews·Emma LindahlHans Jörnvall
Jan 25, 2014·Diabetes/metabolism Research and Reviews·Patrizia Luppi, Peter Drain
Apr 3, 2008·Experimental Diabetes Research·Claire E Hills, Nigel J Brunskill
Jan 7, 2015·Pharmacological Reports : PR·Marwa M Safar, Rania M Abdelsalam
Feb 5, 2015·Diabetes Research and Clinical Practice·John Wahren, Charlotte Larsson
Mar 24, 2012·Diabetes·John WahrenAnders A F Sima
Jun 3, 2016·Journal of Internal Medicine·P Luppi, P Drain
Dec 21, 2016·Journal of Internal Medicine·J Wahren
Jan 17, 2008·American Journal of Alzheimer's Disease and Other Dementias·Behnam SabayanAfshin Borhanihaghighi
Sep 20, 2016·Journal of Internal Medicine·N J Brunskill
Dec 14, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Lina NordquistA Erik G Persson
Aug 1, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jennifer P RichardsRandy S Sprague
Sep 25, 2014·American Journal of Physiology. Endocrinology and Metabolism·Gina L C YostenJohn Wahren
Nov 2, 2018·BMC Complementary and Alternative Medicine·Guohua YuYuanyuan Shi
Jun 27, 2020·Biomedit︠s︡inskai︠a︡ khimii︠a︡·O N Poteryaeva, I F Usynin
Jan 2, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jennifer P RichardsRandy S Sprague
Feb 27, 2009·Clinical Science·Claire E Hills, Nigel J Brunskill
Jun 15, 2007·The Journal of Biological Chemistry·Vijay YanamadalaBradley M Denker
Oct 13, 2019·Life Sciences·Habib YaribeygiAmirhossein Sahebkar
Oct 23, 2010·Archives of Biochemistry and Biophysics·Mohamed M El-SeweidyOlfat G Shaker

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis