C. quinoa and S. hispanica L. Seeds Provide Immunonutritional Agonists to Selectively Polarize Macrophages.

Cells
Maša SrdićJ M Laparra

Abstract

Diet-related immunometabolic-based diseases are associated with chronic inflammation in metabolic tissues, and infiltrated macrophages have been suggested as mediators for tissue- damaging inflammation. Growing evidence implicates Chenopodium quinoa and Salvia hispanica L. as important contributors to immunonutritional health. However, the functional roles of the immunonutritional protease inhibitors (PPIs) found in these crops on the macrophages' metabolic and phenotypic adaptation remain to be elucidated. The salt soluble fraction of proteins was extracted and analyzed confirming the presence of 11S and 2S albumin. The <30 kDa fraction of the extract from both crops was subjected to simulated gastrointestinal digestion, where (RP-LC-MS/MS analyses) polypeptides from 2S-type of proteins were found, along with the 2S albumin (13 kDa) for S. hispanica in the bioaccessible fraction (BAF). Using human-like macrophage cells to deepen our understanding of the modulatory effects of this BAF, FACS analyses revealed their potential as TLR4 agonists, favoring increased phenotypic CD68/CD206 ratios. The results of mitochondrial stress tests showed that cells increased oxygen consumption rates and non-mitochondrial respiration, confirming...Continue Reading

References

Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Feb 13, 2002·The Journal of Cell Biology·Frédéric MallardLudger Johannes
Nov 30, 2006·Biological Chemistry·Hiroshi Kobayashi
May 28, 2009·Science Signaling·Roy L Silverstein, Maria Febbraio
Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Di WangLinrong Lu
Sep 4, 2010·Journal of the Science of Food and Agriculture·Antonio Vega-GálvezEnrique A Martínez
Dec 27, 2011·Trends in Biochemical Sciences·Monique Gangloff
Apr 28, 2012·Biochemical and Biophysical Research Communications·Kenneth R FeingoldCarl Grunfeld
Aug 23, 2012·Traffic·Amra MrakovicRoberto J Botelho
Dec 5, 2012·The Journal of Experimental Medicine·Yvonne JunkerDetlef Schuppan
Dec 18, 2012·Journal of Agricultural and Food Chemistry·María R Sandoval-Oliveros, Octavio Paredes-López
Sep 26, 2013·Mediators of Inflammation·Francisco J RiosS Jancar
Jan 25, 2014·Free Radical Biology & Medicine·Mari-Luz MorenoJuan Sastre
Sep 18, 2014·Frontiers in Immunology·Silvia Galván-Peña, Luke A J O'Neill
Apr 22, 2015·International Journal of Biological Sciences·Ruth AlonsoLuis Carrasco
Jul 5, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J M LaparraA Cilla
Feb 26, 2016·Cellular & Molecular Immunology·Cynthia Ju, Frank Tacke
Jun 17, 2016·Molecular & Cellular Oncology·Huafeng Zhang
Sep 16, 2016·Cellular and Molecular Life Sciences : CMLS·Vincent A van der MarkCoen C Paulusma
Oct 13, 2016·Cell Reports·Jan Van den BosscheMenno P J de Winther
Dec 23, 2016·Frontiers in Pharmacology·Sandhya Srikanth, Zhong Chen
Sep 29, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Motofumi KumazoeHirofumi Tachibana
Nov 30, 2018·Nature Reviews. Gastroenterology & Hepatology·Konstantin KazankovHenning Grønbæk
Dec 6, 2019·Journal of Translational Medicine·Benedetta M MottaPeter P Pramstaller

❮ Previous
Next ❯

Methods Mentioned

BETA
FACS
Assay
ELISA
PCR
electrophoresis

Software Mentioned

Statgraphics© Plus
Proteome Discoverer
FACS Diva

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here