C-terminal extension of truncated recombinant proteins in Escherichia coli with a 10Sa RNA decapeptide.

The Journal of Biological Chemistry
G F TuR J Simpson

Abstract

When murine interleukin-6 is overexpressed in Escherichia coli, a small population of molecules exhibits a novel C-terminal modification. Peptide mapping, electrospray ionization-mass spectrometry, and automated N- and C-terminal sequencing identified a peptide ("tag" peptide), -Ala-Ala-Asn-Asp-Glu-Asn-Tyr-Ala-Leu-Ala-Ala-COOH, encoded by a small metabolically stable RNA of E. coli (10Sa RNA) attached to truncated C termini of the recombinant protein. A mutant strain of E. coli in which the chromosomal 10Sa RNA gene (ssrA) is disrupted does not produce this C-terminal modification, confirming that the tag peptide originates from the ssrA gene.

References

Jul 2, 1979·Molecular & General Genetics : MGG·B K Ray, D Apirion
Feb 1, 1992·Molecular Microbiology·M J Brumlik, D G Storey
Sep 1, 1991·Molecular & General Genetics : MGG·B K Oh, D Apirion
Nov 1, 1989·Molecular Microbiology·A K Chauhan, D Apirion
Oct 3, 1989·Biochemistry·L R PaborskyJ R Swartz
Feb 1, 1988·European Journal of Immunology·J Van SnickR J Simpson
Nov 30, 1988·Biochemical and Biophysical Research Communications·R J SimpsonJ Van Snick
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y KomineH Inokuchi
Jan 15, 1994·European Journal of Biochemistry·A M JespersenH H Sørensen

❮ Previous
Next ❯

Citations

Nov 26, 2005·Archives of Microbiology·Sandrine BraudPhilippe Mazodier
Nov 13, 2008·Systems and Synthetic Biology·Michael S AllenMichael L Simpson
Jun 22, 2005·Journal of Molecular Biology·Natalia IvanovaMåns Ehrenberg
Sep 10, 2002·Journal of Molecular Biology·Diane O'Handley, Hiroshi Nakai
Jul 9, 1999·Biochimica Et Biophysica Acta·B FeldenJ F Atkins
Jan 1, 1996·Biochimie·B FeldenR F Gesteland
Apr 12, 2002·Gene·Pavel V BaranovJohn F Atkins
Dec 10, 2002·Current Opinion in Chemical Biology·Alexander HüttenhoferJean Pierre Bachellerie
Apr 6, 2002·Current Opinion in Microbiology·Jeffrey H Withey, David I Friedman
Jul 18, 2009·Nature Reviews. Microbiology·Janine KirsteinKürşad Turgay
Jul 26, 2002·Molecular Microbiology·Justine CollierPhilippe Bouloc
Oct 15, 2008·Proceedings of the National Academy of Sciences of the United States of America·Eyal Gur, Robert T Sauer
Jun 14, 2008·The Journal of Biological Chemistry·Mark Lies, Michael R Maurizi
Feb 21, 1998·Nucleic Acids Research·K P Williams, D P Bartel
Dec 10, 1998·Nucleic Acids Research·K P Williams
Mar 28, 2002·Nucleic Acids Research·Kyoko Hanawa-SuetsuguAkira Muto
Jul 26, 2002·Nucleic Acids Research·Dean LaslettSiv Andersson
Aug 6, 2004·Nucleic Acids Research·Takayuki KonnoHyouta Himeno
Jun 24, 2005·Nucleic Acids Research·Natalia IvanovaLovisa Holmberg Schiavone
Oct 6, 2005·Nucleic Acids Research·Krisana AsanoHyouta Himeno
Feb 14, 2006·Nucleic Acids Research·J Ross BuchanIan Stansfield
Mar 17, 2012·Nucleic Acids Research·Gabriel RosenblumBarry S Cooperman
Mar 1, 2008·Protein Engineering, Design & Selection : PEDS·Bryan D Smith, Ronald T Raines
May 6, 2003·Genes & Development·Saskia B NeherTania A Baker
Jul 24, 2007·Applied and Environmental Microbiology·Itzhak MizrahiEliora Z Ron
Feb 17, 2009·Journal of Bacteriology·Sarita Ahlawat, Donald A Morrison
Jun 8, 2001·Journal of Bacteriology·T Wiegert, W Schumann
Jun 12, 2002·Journal of Bacteriology·Shunji NakanoPeter Zuber
Jan 4, 2003·Journal of Bacteriology·Kenneth C Keiler, Lucy Shapiro
Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Franz Narberhaus
Jun 17, 2005·BMC Molecular Biology·Jody BurksJacek Wower

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.