C-terminal interactions of apolipoprotein E4 respond to the postprandial state.

Journal of Lipid Research
Sarada D TetaliJohn C Rutledge

Abstract

Increased triglyceride-rich lipoproteins (TGRLs) in the postprandial state are associated with atherosclerosis. We investigated whether the postprandial state induced structural changes at the apolipoprotein E4 (apoE4) C terminus, its principal lipid binding domain, using electron paramagnetic resonance (EPR) spectroscopy of a site-directed spin label attached to the cysteine of apoE4-W264C. Spin coupling between labels located in the C termini was followed after mixing with preprandial and postprandial human plasma samples. Our results indicate that postprandial plasma triggers a reorganization of the protein such that the dipolar broadening is diminished, indicating a reduction in C-terminal interaction. The loss of spectral broadening was directly correlated with an increase in postprandial plasma triglycerides and was reduced with delipidated plasma. The spin-labeled apoE4 displayed a lipid preference of VLDL > LDL > HDL in the preprandial and postprandial states. The apoE4 shift to VLDL during the postprandial state was accompanied by a loss in spectral broadening of the protein. These findings suggest that apoE4 associated with LDL maintains self-association via its C terminus and that this association is diminished in VL...Continue Reading

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Citations

Feb 18, 2016·Acta Neuropathologica·Leon M TaiGuojun Bu
Oct 18, 2011·Biochimica Et Biophysica Acta·Eric A Schwartz, Peter D Reaven
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Dec 29, 2020·Biochimica Et Biophysica Acta. Biomembranes·Annapoorna R Sapuri-ButtiAtul N Parikh

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