C-terminal truncations of the yeast nucleoporin Nup145p produce a rapid temperature-conditional mRNA export defect and alterations to nuclear structure.

Molecular and Cellular Biology
T C DockendorffCharles N Cole

Abstract

A screen for temperature-sensitive mutants of Saccharomyces cerevisiae defective in nucleocytoplasmic trafficking of poly(A)+ RNA has identified an allele of the NUP145 gene, which encodes an essential nucleoporin. NUP145 was previously identified by using a genetic synthetic lethal screen (E. Fabre, W. C. Boelens, C. Wimmer, I. W. Mattaj, and E. C. Hurt, Cell 78:275-289, 1994) and by using a monoclonal antibody which recognizes the GLFG family of vertebrate and yeast nucleoporins (S. R. Wente and G. Blobel, J. Cell Biol. 125:955-969, 1994). Cells carrying the new allele, nup145-10, grew at 23 and 30 degrees C but were unable to grow at 37 degrees C. Many cells displayed a modest accumulation of poly(A)+ RNA under permissive growth conditions, and all cells showed dramatic and rapid nuclear accumulation of poly(A)+ RNA following a shift to 37 degrees C. The mutant allele contains a nonsense codon which truncates the 1,317-amino-acid protein to 698 amino acids. This prompted us to examine the role of the carboxyl half of Nup145p. Several additional alleles that encode C-terminally truncated proteins or proteins containing internal deletions of portions of the carboxyl half of Nup145p were constructed. Analysis of these mutants i...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·S R WenteG Blobel
Jun 26, 1992·Cell·J E HinshawR A Milligan
Dec 1, 1991·Journal of Structural Biology·M Jarnik, U Aebi
Jan 1, 1991·Methods in Enzymology·F Sherman
May 1, 1987·The Journal of Cell Biology·C M SnowL Gerace
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Mar 1, 1994·The Journal of Cell Biology·A JarmolowskiI W Mattaj
Apr 21, 1995·Cell·E Izaurralde, I W Mattaj
Sep 1, 1995·The Journal of Cell Biology·P GrandiE C Hurt
Sep 1, 1995·The Journal of Cell Biology·M E Hurwitz, G Blobel
Jan 1, 1995·Annual Review of Biochemistry·L I Davis
Apr 1, 1995·Molecular Biology of the Cell·R SchneiterA M Tartakoff
Jun 1, 1995·Trends in Genetics : TIG·V Doye, E C Hurt
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·L F PembertonG Blobel
Jun 1, 1994·Current Opinion in Cell Biology·E Fabre, E C Hurt
Oct 1, 1994·The Journal of Cell Biology·A M BogerdL I Davis
Apr 1, 1994·Current Opinion in Genetics & Development·D J ElliottM Rosbash
Nov 1, 1993·The Journal of Cell Biology·M P Rout, G Blobel
Dec 1, 1995·The Journal of Cell Biology·J D AitchisonM P Rout
Sep 20, 1996·Cell·V W PollardG Dreyfuss
Oct 1, 1994·Trends in Cell Biology·M P Rout, S R Wente

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Citations

Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y LiuA M Tartakoff
Nov 5, 1999·The Journal of Biological Chemistry·M T TeixeiraB Dujon
Apr 19, 2011·Annual Review of Biochemistry·André HoelzGünter Blobel
Jul 22, 2018·Biochemical Society Transactions·David J Thaller, C Patrick Lusk
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hyuk-Soo SeoAndré Hoelz
Sep 4, 2014·Genes·Amandine Bonnet, Benoit Palancade
Feb 1, 2017·Cellular and Molecular Life Sciences : CMLS·Flavie CourjolMathieu Gissot
Oct 1, 2003·Molecular and Cellular Biology·Jost EnningaBeatriz M A Fontoura
Oct 16, 2004·Molecular and Cellular Biology·Joanna KufelDavid Tollervey
Sep 5, 2009·PLoS Computational Biology·Timothy R LezonIvet Bahar
Jan 4, 2008·Molecular and Cellular Biology·Karen K ResendesDouglass J Forbes
Jun 1, 1997·Current Opinion in Cell Biology·V Doye, E Hurt
Nov 13, 2008·Developmental Cell·Papia ChakrabortyBeatriz M A Fontoura
Nov 14, 1997·Genes & Development·C A SaavedraC N Cole

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