C-termini are essential and distinct for nucleic acid binding of human NABP1 and NABP2

Biochimica Et Biophysica Acta
Venkatasubramanian VidhyasagarYuliang Wu


Human Nucleic Acid Binding Protein 1 and 2 (hNABP1 and 2; also known as hSSB2 and 1, respectively) are two newly identified single-stranded (ss) DNA binding proteins (SSB). Both NABP1 and NABP2 have a conserved oligonucleotide/oligosaccharide-binding (OB)-fold domain and a divergent carboxy-terminal domain, the functional importance of which is unknown. Recombinant hNABP1/2 proteins were purified using affinity and size exclusion chromatography and their identities confirmed by mass spectrometry. Oligomerization state was checked by sucrose gradient centrifugation. Secondary structure was determined by circular dichroism spectroscopy. Nucleic acid binding ability was examined by EMSA and ITC. Both hNABP1 and hNABP2 exist as monomers in solution; however, hNABP2 exhibits anomalous behavior. CD spectroscopy revealed that the C-terminus of hNABP2 is highly disordered. Deletion of the C-terminal tail diminishes the DNA binding ability and protein stability of hNABP2. Although both hNABP1 and hNABP2 prefer to bind ssDNA than double-stranded (ds) DNA, hNABP1 has a higher affinity for ssDNA than hNABP2. Unlike hNABP2, hNABP1 protein binds and multimerizes on ssDNA with the C-terminal tail responsible for its multimerization. Both hNAB...Continue Reading


Jul 20, 2016·Biochimica Et Biophysica Acta·Monica Rienzo, Amelia Casamassimi


Jul 1, 1992·Molecular and Cellular Biology·C KimM S Wold
Jan 1, 1994·Annual Review of Biochemistry·Timothy M Lohman, M E Ferrari
Jun 1, 1994·Molecular and Cellular Biology·L J Blackwell, James A Borowiec
Jun 27, 2000·Bioinformatics·L J McGuffinD T Jones
Jun 7, 2003·Science·Lee Zou, Stephen J Elledge
Jun 16, 2004·Cellular and Molecular Life Sciences : CMLS·C GiglioneT Meinnel
Jun 25, 2004·Protein Science : a Publication of the Protein Society·You-Di LiaoShu-Ting Chang
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·H Jane Dyson, Peter E Wright
Mar 23, 2005·Trends in Biotechnology·Quinn Lu
Mar 15, 2006·The Biochemical Journal·Hong Soon KangAnton M Jetten
Aug 29, 2006·Nucleic Acids Research·Ellen FanningAndrew R Nager
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rachel William AnanthaJames A Borowiec
Oct 22, 2008·Critical Reviews in Biochemistry and Molecular Biology·Robert D SheredaJames L Keck
May 23, 2009·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Eszter Hazy, Peter Tompa
Jul 17, 2009·The Journal of Biological Chemistry·Yongjiang LiWeidong Wang
Aug 18, 2009·Molecular Cell·Jun HuangJunjie Chen
Sep 30, 2009·The Journal of Cell Biology·Jeffrey R SkaarMichele Pagano
Nov 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jintao LiuCarlos J Camacho
Jan 27, 2010·Biochimica Et Biophysica Acta·Bin XueVladimir N Uversky
Apr 3, 2010·The Journal of Biological Chemistry·Alexander G KozlovTimothy M Lohman
Jun 3, 2010·Critical Reviews in Biochemistry and Molecular Biology·Rachel Litman Flynn, Lee Zou
Mar 2, 2011·The Journal of Biological Chemistry·Yu ZengPrakash Kulkarni
Mar 13, 2012·Annual Review of Biophysics·Taekjip HaTimothy M Lohman
Jun 8, 2012·Proteins·Mark A SeegerSarah E Rice
Nov 28, 2012·Cell Research·Shuangbing XuTiebang Kang
Aug 30, 2013·Journal of Cell Science·Feng ZhangXiaochun Yu
Sep 12, 2013·Journal of Molecular Biology·Edwin AntonyTimothy M Lohman
Mar 19, 2014·Cell Reports·Wendan RenHaiwei Song
Apr 29, 2015·Nucleic Acids Research·Ananya KarSandeep Saxena

Related Concepts

Centrifugation, Density Gradient
NABP1 protein, mouse
Carboxy-Terminal Amino Acid
DNA Repair
Regulation of Biological Process
Leptin receptor
NABP1 gene
Gene Deletion Abnormality

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.