C-X-C motif chemokine ligand 10 produced by mouse Sertoli cells in response to mumps virus infection induces male germ cell apoptosis

Cell Death & Disease
Qian JiangDaishu Han

Abstract

Mumps virus (MuV) infection usually results in germ cell degeneration in the testis, which is an etiological factor for male infertility. However, the mechanisms by which MuV infection damages male germ cells remain unclear. The present study showed that C-X-C motif chemokine ligand 10 (CXCL10) is produced by mouse Sertoli cells in response to MuV infection, which induces germ cell apoptosis through the activation of caspase-3. CXC chemokine receptor 3 (CXCR3), a functional receptor of CXCL10, is constitutively expressed in male germ cells. Neutralizing antibodies against CXCR3 and an inhibitor of caspase-3 activation significantly inhibited CXCL10-induced male germ cell apoptosis. Furthermore, the tumor necrosis factor-α (TNF-α) upregulated CXCL10 production in Sertoli cells after MuV infection. The knockout of either CXCL10 or TNF-α reduced germ cell apoptosis in the co-cultures of germ cells and Sertoli cells in response to MuV infection. Local injection of MuV into the testes of mice confirmed the involvement of CXCL10 in germ cell apoptosis in vivo. These results provide novel insights into MuV-induced germ cell apoptosis in the testis.

References

Oct 1, 1987·The Journal of Experimental Medicine·A D Luster, J V Ravetch
Jan 1, 1973·Scandinavian Journal of Infectious Diseases·B Bjorvatn
Aug 15, 1997·The Biochemical Journal·G M Cohen
Nov 20, 1997·The Journal of Urology·R CasellaT C Gasser
Nov 26, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·C TsigosG P Chrousos
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·N Dejucq, B Jégou
Mar 1, 2003·Journal of Reproductive Immunology·Mark P Hedger, Andreas Meinhardt
May 30, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·Richard M SharpeJane S Fisher
Mar 18, 2004·Molecular and Cellular Endocrinology·S R BornsteinI Vrezas
Nov 4, 2006·Journal of the Royal Society of Medicine·M MasaraniM Dinneen
Mar 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Gabriele S V CampanellaAndrew D Luster
Jun 24, 2008·Human Reproduction·A Le TortorecN Dejucq-Rainsford
Mar 6, 2009·Microscopy Research and Technique·Vanesa Anabella GuazzoneLivia Lustig
Jan 15, 2010·BJU International·Niall F DavisJohn M Fitzpatrick
Apr 17, 2010·American Journal of Respiratory and Critical Care Medicine·Dina SchneiderUmadevi Sajjan
Oct 1, 2011·Cell Death and Differentiation·T KaufmannP J Jost
Nov 6, 2013·Autoimmunity Reviews·Alessandro AntonelliPoupak Fallahi
Mar 13, 2014·Human Vaccines & Immunotherapeutics·Yan LiangQihan Li
Feb 11, 2015·Medical Science Monitor Basic Research·Kuan LiuXing Wu
Nov 1, 2016·Nature·Jennifer GoveroMichael S Diamond
Mar 7, 2017·Science Advances·Ryuta UrakiErol Fikrig

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Citations

Dec 21, 2018·Andrologia·Weihua LiuDaishu Han
Feb 8, 2020·Physiological Reviews·Anna Le TortorecNathalie Dejucq-Rainsford
Aug 28, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Han WuDaishu Han
Nov 8, 2019·Asian Journal of Andrology·Wei-Hua LiuDai-Shu Han
May 13, 2018·Pathogens·Lucia Regina Cangussu da Silva
Jan 22, 2021·Asian Journal of Andrology·Thiago A TeixeiraJorge Hallak
Mar 9, 2021·Frontiers in Immunology·Ran ChenDaishu Han
Apr 6, 2021·Frontiers in Immunology·Han WuDaishu Han
Jul 2, 2021·Andrology·Amaro N Duarte-NetoJorge Hallak

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Methods Mentioned

BETA
flow cytometry
ELISA
FCS
PCR
protein assay

Software Mentioned

SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis