C14orf166 is a high-risk biomarker for bladder cancer and promotes bladder cancer cell proliferation

Journal of Translational Medicine
Mingkun ChenZike Qin

Abstract

C14orf166 (chromosome 14 open reading frame 166) plays a crucial role in some tumors, but its role in bladder cancer hasn't been explored. We determined C14orf166 expression in uroepithelial cell, bladder cancer cells, normal bladder tissues and bladder cancer tissues using quantitative RT-PCR and western blot, we then analyzed the correlation between C14orf166 expression and clinicopathologic characteristics in a cohort of 149 patients with bladder cancer. Finally we downregulated C14orf166 and determined its role in the proliferation of bladder cancer cell lines using MTT assay, colony formation assay and cell cycle assay. We demonstrated C14orf166 was upregulated in bladder cancer cells and tissues, C14orf166 expression was significantly correlated with larger tumor size (P = 0.001), lymph node involvement (P < 0.001), histological differentiation (P < 0.001), survival time and vital states, and high C14orf166 expression correlated with poor survival, these results suggested C14orf166 served as a high-risk marker for bladder cancer. Knockdown of C14orf166 decreased the proliferation rate and colony formation ability of bladder cancer cells, and arrested cell cycle in G1/S transition. Further analysis showed that C14orf166 kn...Continue Reading

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Citations

Mar 26, 2019·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Jianxu ChenJian Li
Feb 13, 2018·Oncology Letters·Xuebao XiangPengcheng Li

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Methods Mentioned

BETA
PCR
protein assay

Software Mentioned

SPSS

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