C1GALT1 Seems to Promote In Vitro Disease Progression in Ovarian Cancer

International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society
Chih-Hsing ChouMin-Chuan Huang

Abstract

Aberrant glycosylation affects many cellular properties in cancers. The core 1 β1,3-galactosyltransferase (C1GALT1), an enzyme that controls the formation of mucin-type O-glycans, has been reported to regulate hepatocellular and mammary carcinogenesis. This study aimed to explore the role of C1GALT1 in ovarian cancer. C1GALT1 expression was assessed in a public database based on microarray data from 1287 ovarian cancer patients and ovarian cancerous tissues. Lectin blotting and flow cytometry analysis were conducted to detect changes in O-glycans on ovarian cancer cells. Effects of C1GALT1 on cell growth, migration, and sphere formation were analyzed in C1GALT1 knockdown or overexpressing ovarian cancer cells in vitro. Expression of cancer stemness-related genes was analyzed by quantitative reverse transcription polymerase chain reaction. High C1GALT1 expression shows a trend toward association with poor survival in ovarian cancer patients. C1GALT1 modifies O-glycan expression on surfaces and glycoproteins of ovarian cancer cells. Knockdown of C1GALT1 decreased cell growth, migration, and sphere formation of ES-2 and OVTW59-p4 cells. Conversely, overexpression of C1GALT1 promoted such malignant properties of SKOV3 cells. Furthe...Continue Reading

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Citations

Aug 5, 2018·Science China. Life Sciences·Yuming WangLei Huang
Jul 18, 2018·International Journal of Molecular Sciences·Ricardo CoelhoSara Ricardo
Jun 22, 2021·Oncology Letters·Xiaojie SunXiangwei Meng
Aug 29, 2021·Cell & Bioscience·Xiaoxia DongLi Shen

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