C3 Opsonization of Anthrax Bacterium and Peptidoglycan Supports Recognition and Activation of Neutrophils.

Microorganisms
Narcis I PopescuFlorea Lupu

Abstract

Neutrophils are the most abundant innate cell population and a key immune player against invading pathogens. Neutrophils can kill both bacterium and spores of Bacillus anthracis, the causative anthrax pathogen. Unlike interactions with professional phagocytes, the molecular recognition of anthrax by neutrophils is largely unknown. In this study, we investigated the role of complement C3 deposition on anthrax particles for neutrophil recognition of bacterium and/or its cell wall peptidoglycan, an abundant pathogen-associated molecular pattern that supports anthrax sepsis. C3 opsonization and recognition by complement receptors accounted for 70-80% of the affinity interactions between neutrophils and anthrax particles at subphysiologic temperatures. In contrast, C3 supported up to 50% of the anthrax particle ingestion under thermophysiologic conditions. Opsonin-dependent low affinity interactions and, to a lower extent, opsonin-independent mechanisms, provide alternative entry routes. Similarly, C3 supported 58% of peptidoglycan-induced degranulation and, to a lower extent, 23% of bacterium-induced degranulation. Interestingly, an opsonin independent mechanism mediated by complement C5, likely through C5a anaphylatoxin, primes az...Continue Reading

References

Aug 1, 1988·The Journal of Clinical Investigation·B L MyonesG D Ross
Jan 1, 1985·Infection and Immunity·J O'BrienS Leppla
Nov 18, 1994·Science·M MeselsonO Yampolskaya
Nov 5, 1997·Immunity·L B KlicksteinA Nicholson-Weller
May 16, 2001·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·L M GrinbergJ H Smith
Dec 19, 2001·Emerging Infectious Diseases·J A JerniganUNKNOWN Anthrax Bioterrorism Investigation Team
May 1, 2002·JAMA : the Journal of the American Medical Association·Thomas V InglesbyUNKNOWN Working Group on Civilian Biodefense
Jun 28, 2003·Blood·Fumitaka HayashiAndrew D Luster
Oct 13, 2004·Molecular Immunology·Philippe Gasque
Dec 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Silvia BellocchioLuigina Romani
Nov 18, 2005·PLoS Pathogens·Anne Mayer-SchollArturo Zychlinsky
May 13, 2006·Molecular Microbiology·Thomas Candela, Agnès Fouet
Jun 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Matthew A CrawfordGary M Bokoch
Aug 1, 2006·The American Journal of Pathology·Deborah J Stearns-KurosawaShinichiro Kurosawa
Jun 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aurélie CleretJean-Nicolas Tournier
Jan 16, 2008·Infection and Immunity·Patrick SanzAlison D O'Brien
Jan 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Claudia R OlivaJohn F Kearney
May 8, 2008·International Journal of Experimental Pathology·Beth L HahnPeter G Sohnle
Mar 31, 2009·Microbial Pathogenesis·Christopher PremanandanAndrew J Phipps
Jul 30, 2009·Molecular Aspects of Medicine·Mahtab Moayeri, Stephen H Leppla
Mar 2, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Janaki K Iyer, K Mark Coggeshall
Aug 20, 2011·American Journal of Respiratory and Critical Care Medicine·Daniel A SweeneyPeter Q Eichacker
Mar 24, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chunfang GuYi Xu
Jul 21, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dawei SunK Mark Coggeshall
Jun 8, 2013·Journal of Cellular and Molecular Medicine·Kenneth Mark CoggeshallShinichiro Kurosawa
Jul 19, 2013·PloS One·Benjamin PulliJohn W Chen
Oct 25, 2013·Cellular Microbiology·Zachary P WeinerIan J Glomski
Jun 14, 2014·MAbs·Austin W BoeschMargaret E Ackerman
Jul 22, 2015·Annual Review of Microbiology·Mahtab MoayeriShihui Liu
Oct 27, 2015·Frontiers in Microbiology·Pierre L Goossens, Jean-Nicolas Tournier
Jun 14, 2017·The Journal of Experimental Medicine·Christian LoodKeith B Elkon
Nov 28, 2017·Seminars in Cell & Developmental Biology·Anna ErdeiZsuzsa Bajtay
Feb 7, 2018·Frontiers in Cellular and Infection Microbiology·Janet Z LiuVictor Nizet

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Methods Mentioned

BETA
immunodepletion
flow cytometry
density gradient centrifugation
immunodepletions
PMA

Software Mentioned

Adobe Illustrator
Prism
FlowJo

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