C57BL/6N mutation in cytoplasmic FMRP interacting protein 2 regulates cocaine response

Science
Vivek KumarJoseph S Takahashi

Abstract

The inbred mouse C57BL/6J is the reference strain for genome sequence and for most behavioral and physiological phenotypes. However, the International Knockout Mouse Consortium uses an embryonic stem cell line derived from a related C57BL/6N substrain. We found that C57BL/6N has a lower acute and sensitized response to cocaine and methamphetamine. We mapped a single causative locus and identified a nonsynonymous mutation of serine to phenylalanine (S968F) in Cytoplasmic FMRP interacting protein 2 (Cyfip2) as the causative variant. The S968F mutation destabilizes CYFIP2, and deletion of the C57BL/6N mutant allele leads to acute and sensitized cocaine-response phenotypes. We propose that CYFIP2 is a key regulator of cocaine response in mammals and present a framework to use mouse substrains to identify previously unknown genes and alleles regulating behavior.

References

Sep 1, 1993·Brain Research. Brain Research Reviews·T E Robinson, K C Berridge
Mar 17, 2001·Nature Reviews. Neuroscience·E J Nestler
Sep 19, 2001·Genetics·S Sen, G A Churchill
Jun 25, 2002·Journal of Molecular Biology·Raphael GueroisLuis Serrano
Aug 16, 2002·Nature·Sharon EdenMarc W Kirschner
Dec 6, 2002·Nature·UNKNOWN Mouse Genome Sequencing ConsortiumEric S Lander
Dec 6, 2002·Nature·Claire M WadeMark J Daly
Feb 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Scott H SoderlingJohn D Scott
May 2, 2003·Bioinformatics·Karl W BromanGary A Churchill
May 13, 2003·Molecular Psychiatry·T M Tzschentke, W J Schmidt
Mar 17, 2004·Current Opinion in Pharmacology·Peter W Kalivas
Sep 24, 2004·Developmental Biology·Annette SchenckAngela Giangrande
Dec 8, 2006·Nature·Ed S LeinAllan R Jones
Jan 12, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Scott H SoderlingJohn D Scott
Jul 27, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Saïd KourrichMark J Thomas
Sep 27, 2007·Neural Development·Abrar QurashiAngela Giangrande
Dec 17, 2008·Journal of Neurogenetics·Camron D BryantJames A McRoberts
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Jun 16, 2009·Nature Methods·Stephen J PettittWilliam C Skarnes
Jun 19, 2009·Genetics·Allison CoxKarl W Broman
Aug 28, 2009·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·George F Koob, Nora D Volkow
Dec 22, 2009·Bioinformatics·Jared T SimpsonRichard Durbin
Apr 1, 2010·Nature Methods·Ivan A AdzhubeiShamil R Sunyaev
Nov 26, 2010·Nature·Zhucheng ChenMichael K Rosen
May 31, 2011·Nature Genetics·Hyuna YangFernando Pardo-Manuel de Villena
Sep 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Vivek KumarJoseph S Takahashi
Sep 17, 2011·Nature·Thomas M KeaneDavid J Adams
Nov 22, 2011·Nucleic Acids Research·Terry P MaddatuMolly A Bogue
Feb 22, 2012·Genetics·Karen L SvensonGary A Churchill

❮ Previous
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Citations

Jan 24, 2014·Lab Animal·Kara Rosania
Jan 25, 2014·Science·Gary A Churchill
Jul 6, 2014·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Laura J SittigAbraham A Palmer
Dec 9, 2014·Pharmacology, Biochemistry, and Behavior·Adriana HuertasMikhail Y Inyushin
Dec 9, 2015·Genes, Brain, and Behavior·Camron D Bryant, Neema Yazdani
Mar 3, 2015·Genes, Brain, and Behavior·T WiltshireL M Tarantino
Jan 30, 2015·Frontiers in Behavioral Neuroscience·Stacey L Kirkpatrick, Camron D Bryant
Feb 14, 2015·PLoS Genetics·John P DidionFernando Pardo-Manuel de Villena
Aug 16, 2016·Brain : a Journal of Neurology·Sachin Suresh TiwariKarl Peter Giese
Sep 3, 2016·Scientific Reports·Mohammad Sarowar HossainMasashi Yanagisawa
Nov 5, 2016·Nature·Hiromasa FunatoMasashi Yanagisawa
Oct 25, 2014·Experimental Animals·Kazuyuki MekadaAtsushi Yoshiki
Feb 4, 2014·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Allison R RogalaFernando Pardo-Manuel de Villena
Dec 2, 2017·Genes, Brain, and Behavior·D G AshbrookR W Williams
Apr 7, 2017·Disease Models & Mechanisms·Amelie Baud, Jonathan Flint
Feb 18, 2018·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·R Christopher PierceMathieu E Wimmer
Dec 21, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Liang ZhaoThaddeus S Nowak
Mar 13, 2019·G3 : Genes - Genomes - Genetics·John R ShorterFernando Pardo-Manuel de Villena
Jun 13, 2015·Nature·Kent LloydTerry Magnuson
Jun 20, 2019·Genes, Brain, and Behavior·Susan E MaloneyJoseph D Dougherty
Jul 5, 2019·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Sophie Laguesse, Dorit Ron
Aug 8, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Elizabeth P NewberryNicholas O Davidson
May 7, 2019·Genes, Brain, and Behavior·Marleen H M de GrootJoseph S Takahashi
Aug 29, 2019·Anais Da Academia Brasileira De Ciências·Jean-François Bach
Apr 12, 2017·Current Protocols in Neuroscience·Clarissa C ParkerElissa J Chesler
May 14, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Qiu T RuanCamron D Bryant
Aug 31, 2020·Alcoholism, Clinical and Experimental Research·Matthew C HartmannAlan M Rosenwasser

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