C6 glioma cells differentiated by retinoic acid overexpress the glutamate transporter excitatory amino acid carrier 1 (EAAC1)

Neuroscience
M G BianchiOvidio Bussolati

Abstract

The transport of excitatory amino acids (EAA) in CNS is performed by a family of high affinity, sodium dependent carriers. One of these transporters, excitatory amino acid carrier 1 (EAAC1), is known to be regulated by several mechanisms that modify carrier abundance on the plasma membrane. Much less is known on EAAC1 regulation at the level of gene expression. Here we report that, in C6 rat glioma cells, a line recently described to contain neural stem-like cells, EAAC1 is markedly induced by all trans-retinoic acid (ATRA), a well known differentiating agent. Consistently, ATRA stimulates EAA transport, with the maximal effect observed at concentrations>or=1 microM. After 4 days of treatment with 10 microM ATRA, the transport Vmax is fivefold enhanced, Slc1a1 mRNA is increased by 400% compared with control, EAAC1 carrier is sixfold overexpressed and the C6 culture is greatly enriched of cells with bipolar morphology strongly positive for EAAC1 immunoreactivity. Compared with untreated cells, ATRA-treated C6 cells express less Slc1a3 mRNA, for the transporter GLAST, but significantly higher levels of Slc1a2 mRNA, for the transporter GLT-1, although no expression of either protein is detected with Western blot in both untreated ...Continue Reading

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Citations

Jul 16, 2013·Cellular and Molecular Neurobiology·Zhe ShiKe Wang
Oct 29, 2013·Cellular and Molecular Life Sciences : CMLS·Massimiliano G BianchiOvidio Bussolati
Apr 8, 2014·Neurochemical Research·Mery Stéfani Leivas PereiraDiogo Losch de Oliveira
Dec 15, 2015·Brain and Behavior·Chuan-Chuan ChaoChung-Liang Chien
Dec 1, 2009·Neurosurgery Clinics of North America·Jianfei JiJohn S Yu
May 20, 2009·Biochimica Et Biophysica Acta·Massimiliano G BianchiOvidio Bussolati
Nov 15, 2008·Journal of Pharmacological Sciences·Koji AoyamaToshio Nakaki
Jun 17, 2014·Chemical Research in Toxicology·Bianca Maria RotoliEnrico Bergamaschi
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Jun 21, 2015·The Journal of Biological Chemistry·Ha-Nul YuRan-Sook Woo
May 16, 2021·Neuropharmacology·Ada G Rodríguez-Campuzano, Arturo Ortega

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