PMID: 9536889Apr 16, 1998Paper

CA 19-9 ELISA test: a new method for studying mucus changes in tears

The British Journal of Ophthalmology
C GarcherJ Bara

Abstract

This study investigated mucus changes in the tears in various eye conditions using impression cytology. The quantity of mucins was measured by enzyme linked immunosorbent assay (ELISA) using the tumour marker CA 19-9. This assay quantifies the sialylated Lewis(a) structure mainly associated with ocular mucins. Impression cytology was performed using a cellulose nitrate membrane, on 53 healthy patients, 50 glaucoma patients treated with beta blockers, 24 patients suffering from dry eye syndrome, and 45 contact lens wearers. The tear film glycoproteins were eluted and CA 19-9 was measured. CA 19-9 content expressed as kilo units (kU) per microgram of tears was significantly decreased in dry eye syndrome (25.8 kU (SD 17.3)/microgram) (p < 0.05), glaucoma patients over 60 years (28.9 (19.5) kU/microgram) (p < 0.05), and contact lens wearers (28.4 kU (18)/microgram) (p < 0.05), when compared with healthy individuals (39.4 kU (22.2)/microgram). Impression cytology can be regarded as a valuable method for obtaining samples of glycoconjugates of mucin. The decrease of sialylated chains observed with this method confirms the hypothesis that some quantitative changes in the tear film may be encountered in ocular surface disorders.

Associated Clinical Trials

References

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Citations

Apr 27, 2004·Experimental Eye Research·Margarita CalongeJosé M Herreras
Jul 25, 2013·Experimental Eye Research·Alison M McDermott
Jul 25, 2013·Experimental Eye Research·Nadine von Thun Und Hohenstein-BlaulFranz H Grus
Sep 23, 1998·Australian and New Zealand Journal of Public Health·P M LivingstonH R Taylor
Jul 8, 2014·The Ocular Surface·Bernardo Yañez-SotoChristopher J Murphy
Jan 12, 2007·The Ocular Surface·Ilene K GipsonPablo Argüeso
Jan 29, 2010·The Ocular Surface·Ana Guzman-Aranguez, Pablo Argüeso
Apr 3, 2007·Experimental Eye Research·Sandra Spurr-MichaudIlene Gipson
Dec 25, 2016·Histochemistry and Cell Biology·Anthony Corfield
Jul 23, 1999·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·M Kurpakus WheaterL D Hazlett
Aug 5, 2017·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Maureen BoostZhaoran Wang
Aug 31, 2019·Eye & Contact Lens·Pablo Argüeso

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