Ca-NIR: a ratiometric near-infrared calcium probe based on a dihydroxanthene-hemicyanine fluorophore

Chemical Communications : Chem Comm
Mayeul CollotAndrey S Klymchenko

Abstract

Fluorescent calcium probes are essential tools for studying the fluctuation of calcium ions in cells. Herein, we developed Ca-NIR, the first ratiometric calcium probe emitting in the near infrared region. This probe arose from the fusion of a BAPTA chelator and a dihydroxanthene-hemicyanine fluorophore. It is efficiently excited with common 630-640 nm lasers and displays two distinct emission bands depending on the calcium concentration (Kd= ∼8 μM). The physicochemical and spectroscopic properties of Ca-NIR allowed for ratiometric imaging of calcium distribution in live cells.

References

Mar 3, 2005·Journal of the American Chemical Society·Easwaran ArunkumarJörg Daub
Dec 18, 2007·Cell·David E Clapham
Aug 13, 2011·Chemical Communications : Chem Comm·Akihiro MatsuiKoji Suzuki
Dec 23, 2011·Biology of the Cell·Jean-Pierre Mauger
Jul 24, 2012·Journal of the American Chemical Society·Mayeul CollotJean-Maurice Mallet
Jul 24, 2012·Journal of the American Chemical Society·Lin YuanSasa Zhu
Mar 13, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Keitaro UmezawaKoji Suzuki
Apr 1, 2014·Biochimica Et Biophysica Acta·Martin OheimMayeul Collot
Oct 16, 2014·Chemical Society Reviews·Jun YinJuyoung Yoon
Dec 23, 2014·Nature Neuroscience·Yo OtsuSerge Charpak
Mar 7, 2015·Chemical Society Reviews·Graham R C HamiltonJohn F Callan
Apr 1, 2015·ELife·Mayeul CollotJean-Maurice Mallet
Jul 7, 2015·Organic & Biomolecular Chemistry·Jean-Alexandre Richard
Jun 2, 2016·Chemistry : a European Journal·Binglin SuiKevin D Belfield

❮ Previous
Next ❯

Citations

Dec 15, 2017·Chemical Communications : Chem Comm·Hiroaki OgasawaraShigehiro Yamaguchi
Jul 16, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Sophie BouMayeul Collot
Mar 14, 2021·Journal of Photochemistry and Photobiology. B, Biology·Hang LiLing Qiu
Jul 13, 2021·Biochemistry·Xinqi ZhouEvan W Miller
Aug 29, 2019·ACS Omega·Changyou ZhuXiaojiang Xie
Dec 9, 2021·Biomedical Materials·Haoying HuangChunyan Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.