PMID: 2992446May 1, 1985

Ca overload and the action of calcium sensitive proteases, phospholipases and prostaglandin E2 in myocardial cell degradation

Basic Research in Cardiology
T Toyo-okaT Masaki

Abstract

The mechanism of Ca overload-induced myocardial cell injury under hypoxia was examined for the involvement of calcium-activated neutral proteases (CANP), calcium-dependent phospholipases (CDP) or prostaglandins with measuring 45Ca entry, intake of biologically inert dye, nigrosin, into the cultured myocytes, as was useful for the quantification of sarcolemma permeability, and the release of creatine phosphokinase (CPK) to the culture medium. A Ca channel blocker, verapamil (1 and 10 micrograms/ml) or a Ca ionophore, A23187 (0.5 to 4 micrograms/ml) dose-dependently decreased or increased both the Ca entry and nigrosin intake in accordance with the CPK release. Furthermore, the inhibitors against CANP, NCO-700 (2 and 20 micrograms/ml) that was demonstrated to permeate sarcolemma using 14C-labelled reagent, against CDP, mepacrine (1 and 10 micrograms/ml) or against cyclooxygenase, indomethacin (1 and 10 micrograms/ml) caused no effect on the Ca entry, nigrosin intake nor CPK release under hypoxia. These results suggest that the Ca overdose into the myocardial cells potentiates their injury and it is not primarily related to the activation of CANP, CDP nor cyclooxygenase.

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J M HuangM Bacaner
Mar 19, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Soyeon LimKi-Chul Hwang
Jun 21, 2005·Pharmacology & Therapeutics·Tomie KawadaT Toyo-oka

Citations

Aug 1, 1979·Journal of Molecular and Cellular Cardiology·T Toyo-oka, T Masaki
May 30, 1978·Biochemical and Biophysical Research Communications·T Toyo-okaT Masaki
Nov 1, 1976·Cardiovascular Research·W G NaylerA Slade
Mar 21, 1980·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·S Ebashi
Jan 1, 1981·Journal of Biochemistry·M KurodaT Masaki
Feb 1, 1980·Journal of Biochemistry·T Toyo-okaT Masaki
Aug 1, 1958·Experimental Cell Research·J P KALTENBACHW B LYONS

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Calpain II
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