PMID: 11328609May 1, 2001Paper

Ca(2+)- and myristoylation-dependent association of calcineurin with phosphatidylserine

Journal of Biochemistry
B A Perrino, B A Martin

Abstract

It has been proposed that N-terminal myristoylation of calcineurin B is necessary for the membrane association of calcineurin. We tested the effects of Ca(2+) and myristoylation on the binding of calcineurin B alone or heterodimeric calcineurin to phosphatidylserine or phosphatidylcholine vesicles. In the presence of excess phosphatidylserine, 50-60% of total calcineurin associated with phosphatidylserine in a Ca(2+)-sensitive manner. Calcineurin did not associate with phosphatidylcholine. Calcineurin containing both the alpha and beta catalytic subunit isoforms bound to phosphatidylserine. Calmodulin interfered with the association of calcineurin with phosphatidylserine. In the presence of Ca(2+), myristoylated calcineurin B alone did not bind to phosphatidylcholine but did bind to phosphatidylserine, although to a lesser extent than the calcineurin heterodimer. Non-myristoylated calcineurin B alone, or calcineurin containing non-myristoylated calcineurin B did not associate with phosphatidylserine in the presence of Ca(2+). These results indicate: (i) Both isoforms of calcineurin bind to phosphatidylserine. (ii) A phospholipid binding site is located on the calcineurin B subunit. (iii) Calcineurin B myristoylation is required...Continue Reading

Citations

Apr 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Matthew G GoldJohn D Scott
Apr 10, 2007·The Journal of Biological Chemistry·Jeff S IsenbergDavid D Roberts
Jul 19, 2006·Progress in Lipid Research·Ponniah SelvakumarRajendra K Sharma
Mar 7, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·John P WilliamsJay M McDonald
Mar 19, 2021·Cellular & Molecular Immunology·Bin WangFangfang Zhou
Sep 10, 2005·Brain Research·Abdulbaki AgbasElias K Michaelis
Jun 23, 2006·Pharmacology & Therapeutics·Alistair T R SimNicole M Verrills

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