Ca2+ accumulation and loss by aberrant endocytic vesicles in sickle erythrocytes

Journal of Cellular Physiology
P WilliamsonR A Schlegel

Abstract

Sickle cells contain internal vesicles which accumulate Ca2+. As shown here, the membrane enclosing the vesicles contains the plasma membrane Ca(2+)-ATPase, or Ca2+ pump, as judged by staining with an antibody directed against the protein. Moreover, the number of cells containing such vesicles increases upon deoxygenation. These findings argue strongly that the vesicles arise by endocytosis from the plasma membrane, and explain how they accumulate Ca2+. When sickle cells are depleted of ATP, Ca2+ is lost from the vesicles, as judged by the disappearance of staining with the Ca2+/membrane probe chlortetracycline (CTC), without a corresponding loss of antibody staining. This loss of Ca2+ can be inhibited by nitrendipine, a Ca2+ channel blocker. These results suggest that the vesicle membrane allows outward passage of Ca2+ by a nitrendipine-sensitive pathway, which can be overcome by the inward-directed activity of the Ca2+ pump of the vesicle membrane. If so, the Ca2+ which vesicles contain is in dynamic equilibrium with the cytoplasm of the sickle erythrocyte.

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Citations

Sep 22, 2010·Pflügers Archiv : European journal of physiology·Michael FöllerStephan M Huber
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·D W KnowlesJ A Chasis
Jun 11, 2016·Blood Cells, Molecules & Diseases·M P Westerman, John B Porter
Dec 25, 2004·Physiological Reviews·Virgilio L Lew, R M Bookchin
Sep 6, 2001·Cell Death and Differentiation·R A Schlegel, P Williamson

Related Concepts

Ca(2+)-Transporting ATPase
Anemia, Sickle Cell
Calcium
Plasma Membrane
Chlortetracycline Sulfate (2: 1)
Endocytosis
Red Blood Cell Count Measurement
Abnormal Red Blood Cell
Intracellular Membranes
Cell Surface Proteins

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