PMID: 11927673Apr 3, 2002Paper

Ca(2+)-activated Cl(-) current in rabbit sinoatrial node cells

The Journal of Physiology
Arie O VerkerkE Etienne Verheijck

Abstract

The Ca(2+)-activated Cl(-) current (I(Cl(Ca))) has been identified in atrial, Purkinje and ventricular cells, where it plays a substantial role in phase-1 repolarization and delayed after-depolarizations. In sinoatrial (SA) node cells, however, the presence and functional role of I(Cl(Ca)) is unknown. In the present study we address this issue using perforated patch-clamp methodology and computer simulations. Single SA node cells were enzymatically isolated from rabbit hearts. I(Cl(Ca)) was measured, using the perforated patch-clamp technique, as the current sensitive to the anion blocker 4,4'-diisothiocyanostilbene-2,2'-disulphonic acid (DIDS). Voltage clamp experiments demonstrate the presence of I(Cl(Ca)) in one third of the spontaneously active SA node cells. The current was transient outward with a bell-shaped current-voltage relationship. Adrenoceptor stimulation with 1 microM noradrenaline doubled the I(Cl(Ca)) density. Action potential clamp measurements demonstrate that I(Cl(Ca)) is activate late during the action potential upstroke. Current clamp experiments show, both in the absence and presence of 1 microM noradrenaline, that blockade of I(Cl(Ca)) increases the action potential overshoot and duration, measured at 20...Continue Reading

References

Oct 1, 1992·The Journal of Physiology·N HagiwaraH Irisawa
Mar 1, 1992·The Journal of General Physiology·A C Zygmunt, W R Gibbons
Nov 1, 1991·Biophysical Journal·R WildersA C van Ginneken
Jun 1, 1991·Journal of Molecular and Cellular Cardiology·S Kawano, M Hiraoka
Feb 1, 1991·Circulation Research·A C Zygmunt, W R Gibbons
Sep 1, 1990·The Journal of Physiology·J C Denyer, H F Brown
Aug 1, 1988·The Journal of General Physiology·R Horn, A Marty
Apr 1, 1989·Circulation Research·G N Tseng, B F Hoffman
Apr 1, 1989·Pflügers Archiv : European journal of physiology·T DoerrW Trautwein
Jun 1, 1994·The Journal of Membrane Biology·J BescondJ Lenfant
Nov 1, 1994·The American Journal of Physiology·A C Zygmunt
Jan 1, 1993·Physiological Reviews·H IrisawaW Giles
Jul 1, 1996·The American Journal of Physiology·B M WolskaR J Solaro
Jul 1, 1996·Cardiovascular Research·J I VandenbergT Powell
Oct 1, 1996·The American Journal of Physiology·D S LindbladW R Giles
Feb 14, 1998·The Journal of General Physiology·E E VerheijckA C van Ginneken
Feb 11, 1998·The Journal of Physiology·A ZazaM Rocchetti
May 12, 1998·The Journal of Physiology·C H Leem, R D Vaughan-Jones
Jun 25, 1998·The American Journal of Physiology·R W JoynerA C Van Ginneken
Jan 14, 1999·The American Journal of Physiology·N J Bramich, H M Cousins
Mar 10, 1999·Progress in Biophysics and Molecular Biology·P KohlD Noble
Jan 5, 2000·Physiological Reviews·J R HumeB Horowitz
Apr 15, 2000·The Journal of Physiology·J HüserS L Lipsius
Jun 16, 2000·The Journal of Physiology·Y K Ju, D G Allen
Sep 7, 2000·Cardiovascular Research·M R BoyettI Kodama
Oct 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·Y G WangR W Joyner
Feb 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·J WuJ P Boineau

❮ Previous
Next ❯

Citations

Feb 16, 2008·Annals of Biomedical Engineering·Julio Gomis-Tena, Javier Saiz
Apr 15, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Linda J Wang, Eric A Sobie
Dec 26, 2003·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Hiroyasu Satoh
Feb 14, 2004·Acta Physiologica Scandinavica·A O VerkerkJ H Ravesloot
Dec 21, 2010·Biochemical and Biophysical Research Communications·Thomas P VacekSuresh C Tyagi
Apr 22, 2014·Journal of Pharmacological Sciences·Victor A MaltsevEdward G Lakatta
Apr 11, 2015·Frontiers in Physiology·Rebecca A Capel, Derek A Terrar
Apr 21, 2009·Journal of Molecular and Cellular Cardiology·Z Maggie HuangDayue Duan
Jun 27, 2006·Journal of Pharmacological Sciences·Victor A MaltsevEdward G Lakatta
Jul 30, 2003·Circulation·Arie O VerkerkE Etienne Verheijck
Jul 11, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yael YanivEdward G Lakatta
Feb 18, 2005·Acta Pharmacologica Sinica·Da-yue DuanJoseph R Hume
Apr 1, 2008·Annals of the New York Academy of Sciences·Edward G LakattaVictor A Maltsev
Jul 16, 2008·Physiological Reviews·Matteo E Mangoni, Joël Nargeot

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Conduction System

The cardiac conduction system is a specialized tract of myocardial cells responsible for maintaining normal cardiac rhythm. Discover the latest research on the cardiac conduction system here.

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.