PMID: 7539843Mar 15, 1995Paper

Ca(2+)-activated K+ channels in isolated type I cells of the neonatal rat carotid body

The Journal of Physiology
C N Wyatt, C Peers

Abstract

1. Ca(2+)-activated K+ (K+Ca) channels in neonatal rat type I carotid body cells were studied using single channel patch clamp techniques. In outside-out patches, using symmetrical 120 mM [K+] solutions, channels were observed with a slope conductance of 190 pS and a reversal potential of 0 mV. Reducing [K+]o to 5 mM shifted the reversal potential as expected for a K(+)-selective channel. 2. With 100 nM Ca2+ bathing the cytosolic aspect of patches, channel activity (number of active channels in a patch x open probability, NPo) increased with depolarization. NPo also increased with increasing 'cytosolic' [Ca2+] at a fixed membrane potential (0 mV). Using outside-out patches, bath application of 20 or 100 nM charybdotoxin reduced NPo by > 85%. These data indicate the presence of K+Ca channels in type I cells. 3. At 0 mV, using solutions of identical composition (1 microM Ca2+ bathing the cytosolic aspect of the channels), NPo was higher in outside-out patches than in inside-out patches. NPo was greatest in recordings using the perforated-vesicle technique. 4. Hypoxia and anoxia were without effect on K+Ca channels in outside-out patches, but caused significant, reversible reductions of NPo in channels recorded in perforated vesic...Continue Reading

Citations

Oct 23, 1997·The Journal of Physiology·E Carpenter, C Peers
Apr 6, 2007·Respiratory Physiology & Neurobiology·Christopher N Wyatt, A Mark Evans
Mar 31, 2010·The Journal of General Physiology·Patricia Ortega-SáenzJosé López-Barneo
Sep 13, 2006·The Journal of General Physiology·Patricia Ortega-SáenzJosé López-Barneo
Feb 22, 2001·Annual Review of Physiology·J Lopez-BarneoP Ortega-Sáenz
Mar 5, 2016·The Journal of Physiology·Ting ZhouHiroaki Matsunami
Sep 3, 2005·Progress in Biophysics and Molecular Biology·S LahiriN R Prabhakar
Jun 9, 2004·The Japanese Journal of Physiology·Naoji FujishiroMasumi Inoue
Jan 28, 2006·The Journal of Physiology·Luc J TeppemaAlbert Dahan
Oct 23, 2009·Annals of the New York Academy of Sciences·José López-BarneoRaquel Gómez-Díaz
Feb 19, 2002·Nature Neuroscience·Ricardo Pardal, José López-Barneo
Feb 10, 2004·Journal of Applied Physiology·José López-BarneoPatricia Ortega-Sáenz
Mar 23, 2004·The Journal of Physiology·M Teresa Pérez-GarcíaJosé Ramón López-López
Dec 8, 2017·The Journal of Physiology·Ryan J Rakoczy, Christopher N Wyatt
Nov 11, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·L HeC Gonzalez
Jan 27, 2006·American Journal of Physiology. Cell Physiology·Fenglian XuAmy Tse
Jul 22, 2006·Experimental Physiology·Paul J Kemp
Dec 21, 2006·The Journal of Biological Chemistry·Christopher N WyattA Mark Evans
Apr 19, 2012·Canadian Journal of Physiology and Pharmacology·Amy TseFrederick W Tse
Sep 26, 2012·Pflügers Archiv : European journal of physiology·Justin R PapreckDonghee Kim
Oct 30, 2013·The Journal of Physiology·Patricia Ortega-SáenzJosé López-Barneo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.