PMID: 9438828Mar 28, 1998Paper

Ca2+-ATPase activity and Ca2+ uptake by sarcoplasmic reticulum in fish heart: effects of thermal acclimation

The Journal of Experimental Biology
E Aho, Matti Vornanen

Abstract

This study was designed to compare the activities of sarcoplasmic (SR) Ca2+-ATPase and Ca2+ uptake in fish and mammalian hearts and to determine whether thermal acclimation has any effect on the function of the cardiac SR in fish. To this end, we measured thapsigargin-sensitive Ca2+-ATPase activity and thapsigargin-inhibitable Ca2+ uptake velocity in crude cardiac homogenates of newborn and adult rats and of two teleost fish (crucian carp and rainbow trout) acclimated to low (4 degrees C) and high (17 degrees C and 24 degrees C for trout and carp, respectively) ambient temperatures. The TG-sensitive Ca2+-ATPase activity was highest in adult rat, and the corresponding activities of cold-acclimated trout, warm-acclimated trout, warm-acclimated carp, cold-acclimated carp and newborn rat were 76, 58, 43, 28 and 23 %, respectively, of that of the adult rat at 25 degrees C. SR Ca2+ uptake velocity, measured using Fura-2 at room temperature (approximately 22 degrees C), was highest in cold-acclimated trout, and the values for adult rat, warm-acclimated trout, newborn rat, warm-acclimated carp and cold-acclimated carp were 93, 56, 24, 21 and 14 % of the uptake velocity of cold-acclimated trout, respectively. When corrected to the body ...Continue Reading

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