Ca2+ channel currents in dorsal root ganglion neurons of P/Q-type voltage-gated Ca2+ channel mutant mouse, rolling mouse Nagoya

Neuroscience Research
N FukumotoIzumi Shibuya

Abstract

The role of the P/Q-type voltage-gated Ca(2+) channels (VGCCs) in release of neurotransmitters involved in nociception is not fully understood. Rolling mouse Nagoya (tg(rol)), a P/Q-type channel mutant mouse, expresses P/Q-type VGCC whose activation curve has a higher half activation potential and a smaller slope factor than the wild type channel. We previously reported that tg(rol) mice showed hypoalgesic responses to noxious stimuli. In this study, we examined the VGCC current in dorsal root ganglion (DRG) neurons by the whole-cell patch-clamp method. Both ω-agatoxin IVA (0.1 μM) and ω-conotoxin GVIA (1 μM) inhibited the VGCC current by about 40-50% in both the homozygous tg(rol) (tg(rol)/tg(rol)) and wild type (+/+) mice. The voltage-activation relationships of the total VGCC current and the ω-agatoxin IVA-sensitive component in the tg(rol)/tg(rol) mice shifted positively compared to the +/+ mice, whereas that sensitive to the ω-conotoxin GVIA was not different between the two genotypes. The time constant of activation of the VGCC current at -20 mV was longer in the tg(rol)/tg(rol) mice than in the +/+ mice. These changes in the properties of the VGCC in the tg(rol)/tg(rol) mouse may reduce the amount of the released neurotr...Continue Reading

References

Apr 22, 1982·Brain Research·B Lynn, S E Carpenter
Jan 1, 1982·Neuroscience·K Herrup, S L Wilczynski
Feb 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J DoyleL Stubbs
Dec 18, 1998·The Journal of Biological Chemistry·M WakamoriK Imoto
Jul 22, 1999·Annals of the New York Academy of Sciences·W A Catterall
Mar 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Francisco J UrbanoRichard W Tsien
Jun 1, 2005·Current Opinion in Neurobiology·Daniela Pietrobon
Jul 27, 2005·Pain·Hideaki AsaiYasuo Sugiura

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Citations

Feb 5, 2014·Toxicon : Official Journal of the International Society on Toxinology·Tomohiko KayanoTadanori Aimi
Mar 13, 2014·Journal of Biochemical and Molecular Toxicology·Xiaozhen PengSongping Liang
Jul 19, 2018·PLoS Computational Biology·Darshan Mandge, Rohit Manchanda
Jan 2, 2020·International Journal of Molecular Sciences·Francesca PrestoriEgidio D'Angelo

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