Ca2+ channel currents in type I carotid body cells of normoxic and chronically hypoxic neonatal rats

Brain Research
C PeersD Bee

Abstract

Whole-cell patch-clamp recordings were used to study voltage-gated Ca2+ channel currents in type I carotid body cells of young rats born and reared in normoxia or in a chronically hypoxic (CH) environment (10% O2). Currents activated at potentials of -40 mV and more positive, and typically peaked at 0 mV in both groups of cells. Steady-state inactivation curves were similar in the two populations. Ca2+ currents were significantly larger in CH type I cells, but this was accounted for by the increased size of CH cells: current density was similar in both cell types. Nifedipine (5 microM) always partially inhibited currents and Bay K 8644 (2-5 microM) always enhanced currents, indicating the presence of L-type channels. In a small number of cells from each group, the N-type channel blocker omega-conotoxin GVIA caused partial, irreversible inhibition, but in most cells was without discernible effect. These results indicate that type I cells possess L-type Ca2+ channels, that N-type are expressed in some cells and that non-L, non-N-type channels are also present. Furthermore, chronic hypoxia does not appear to cause specific adaptive changes in the properties of Ca2+ channels in type I cells.

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Citations

Aug 30, 2000·Respiration Physiology·E B Gauda, E E Lawson
Sep 1, 2000·Respiration Physiology·N R Prabhakar, J L Overholt
Jul 15, 2009·Respiratory Physiology & Neurobiology·Drew C BurlonChristopher N Wyatt
Apr 28, 2004·Biochemical and Biophysical Research Communications·Kristin HudasekIan M Fearon
Jan 16, 2003·The Journal of Physiology·Zoltán MolnárAndrás Spät
Apr 10, 2007·Respiratory Physiology & Neurobiology·Keith J Buckler
Aug 6, 2003·The Journal of Comparative Neurology·Maria C F BunielDiana L Kunze
Jan 9, 2020·American Journal of Physiology. Cell Physiology·Donghee KimCarl White
Oct 18, 2002·Microscopy Research and Technique·Zun-Yi Wang, Gerald E Bisgard
Oct 5, 2018·Frontiers in Physiology·Raúl Pulgar-SepúlvedaFernando C Ortiz
Sep 14, 1999·The American Journal of Physiology·L M SterniJ L Carroll
Feb 10, 2006·Respiratory Physiology & Neurobiology·M J WasickoJ L Carroll

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