Ca2+ channel regulation by transforming growth factor-beta 1 and bone morphogenetic protein-2 in developing mice myotubes

The Journal of Physiology
Lizbeth Mejia-Luna, G Avila

Abstract

In skeletal muscle myogenesis, precursor cells or myoblasts fuse to form multinucleated cells (myotubes), which then further develop into functional muscle. We investigated if the inhibition of myogenesis by transforming growth factor-beta1 (TGF-beta1) and bone morphogenetic protein-2 (BMP-2) involve regulation of voltage-dependent Ca(2+) channels. Primary cultured myoblasts were kept in fusion medium (0-6 days) in either the absence (control conditions) or the presence of 40 pm TGF-beta1 or 5 nm BMP-2. Subsequently, the developing myotubes were transferred to a growth factor-free recording solution, and subjected to whole cell patch-clamp experiments. At day 0, 14% of non-fusing myoblasts exhibited T-current, whereas the L-current was practically absent. Under control conditions, however, the percentage of T- and L-channel-expressing myotubes increased sharply, from 25% at day 1 to approximately 100% at days 2-6. In addition, parallel increases were determined for Ca(2+)-currents density and cell membrane capacitance (C(m)), which is proportional to the size of myotubes. Interestingly, at days 1-2 TGF-beta1 and BMP-2 eliminated the T-current on initial 14% of T-channel-expressing myoblasts. Moreover, at day 6 the growth factor...Continue Reading

References

Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C CognardG Romey
Jun 1, 1988·The Journal of General Physiology·K G Beam, C M Knudson
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J MassaguéB Nadal-Ginard
Nov 1, 1986·The Journal of Cell Biology·E N OlsonC Wilcox
Nov 1, 1969·Experimental Cell Research·A ShainbergD Yaffe
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Nov 1, 1983·The Journal of Cell Biology·D Bar-Sagi, J Prives
Mar 1, 1981·Developmental Biology·J D DavidC A Higginbotham
Feb 1, 1995·The Journal of General Physiology·R T Dirksen, K G Beam
May 8, 1995·Biochimica Et Biophysica Acta·W MelzerH C Lüttgau
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A B KulkarniS Karlsson
Jan 1, 1997·Methods in Cell Biology·K G Beam, C Franzini-Armstrong
Aug 28, 1998·Cytokine & Growth Factor Reviews·M KawabataK Miyazono
Mar 29, 2000·The Journal of General Physiology·G Avila, R T Dirksen
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·P BijlengaL Bernheim
Mar 30, 2001·The Journal of Biological Chemistry·G AvilaR T Dirksen
Feb 28, 2002·Frontiers in Bioscience : a Journal and Virtual Library·Robert T Dirksen
Dec 31, 2002·Physiological Reviews·Edward Perez-Reyes
Jul 3, 2003·Nature Reviews. Genetics·Maura H ParkerMichael A Rudnicki
Aug 20, 2003·The Journal of Bone and Joint Surgery. American Volume·Peter ten DijkeBernard A J Roelen
Sep 10, 2003·Cell Cycle·Grace K Pavlath, Valerie Horsley
Nov 15, 2003·Reproductive Biology and Endocrinology : RB&E·Jennifer C J Chen, David J Goldhamer
Jan 28, 2004·Cells, Tissues, Organs·Valerie Horsley, Grace K Pavlath
Jan 28, 2004·Birth Defects Research. Part C, Embryo Today : Reviews·Rosa Serra, Chenbei Chang
May 1, 1995·Trends in Endocrinology and Metabolism : TEM·M M Matzuk

❮ Previous
Next ❯

Citations

Jan 14, 2011·Pflügers Archiv : European journal of physiology·Roberto Ramos-MondragónGuillermo Avila
Feb 8, 2011·Journal of Muscle Research and Cell Motility·Elske J SchabortCarola U Niesler
Mar 16, 2016·Molecular and Cellular Biology·Chanèle Cyr-DepauwPeter M Siegel
Mar 14, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yuan GuMatthias W Laschke
Aug 21, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hye-Won KangAlexei Bogdanov
Nov 18, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Chuanyong LuRalph S Marcucio
Mar 5, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Giovanni LigrestiRoberto F Nicosia
Jul 6, 2012·Journal of Tissue Engineering and Regenerative Medicine·Angel E MercadoEsmaiel Jabbari
Sep 2, 2020·Connective Tissue Research·Claire YuanRobert W Grange
Jan 8, 2017·Cold Spring Harbor Perspectives in Biology·Michael W PickupHarold L Moses
Feb 25, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Heon-Woo LeeSuk-Won Jin
Sep 19, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Guillermo AvilaCitlalli I Aguilar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.