Ca2+ content and expression of an acidocalcisomal calcium pump are elevated in intracellular forms of Trypanosoma cruzi.

Molecular and Cellular Biology
H G LuR Docampo

Abstract

The survival of a eukaryotic protozoan as an obligate parasite in the interior of a eukaryotic host cell implies its adaptation to an environment with a very different ionic composition from that of its extracellular habitat. This is particularly important in the case of Ca2+, the intracellular concentration of which is 3 orders of magnitude lower than the extracellular value. Ca2+ entry across the plasma membrane is a widely recognized mechanism for Ca2+ signaling, needed for a number of intracellular processes, and obviously, it would be restricted in the case of intracellular parasites. Here we show that Trypanosoma cruzi amastigotes possess a higher Ca2+ content than the extracellular stages of the parasite. This correlates with the higher expression of a calcium pump, the gene for which was cloned and sequenced. The deduced protein product (Tca1) of this gene has a calculated molecular mass of 121,141 Da and exhibits 34 to 38% identity with vacuolar Ca2+-ATPases of Saccharomyces cerevisiae and Dictyostelium discoideum, respectively. The tca1 gene suppresses the Ca2+ hypersensitivity of a mutant of S. cerevisiae that has a defect in vacuolar Ca2+ accumulation. Indirect immunofluorescence and immunoelectron microscopy analys...Continue Reading

References

Jun 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B S Weakley
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J Appleton, D C Morris
Nov 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C ScalaG C Manara
Jan 1, 1991·Parasitology Research·M J Soares, W de Souza
Feb 1, 1991·The Journal of Membrane Biology·E E Strehler
Jan 1, 1991·Physiological Reviews·E Carafoli
Apr 1, 1990·Molecular and Biochemical Parasitology·A LeFurgeyJ J Blum
Mar 1, 1989·The Histochemical Journal·K T Tokuyasu
Oct 1, 1988·Molecular and Biochemical Parasitology·J A DvorakP A Pella
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A V SomlyoA P Somlyo
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T P Hopp, K R Woods
Jan 1, 1984·International Review of Cytology·W de Souza
Nov 1, 1981·The Journal of protozoology·J Williamson, D J McLaren
Feb 15, 1995·The Biochemical Journal·G BenaimR Docampo
Sep 15, 1995·The Biochemical Journal·R DocampoS N Moreno
Dec 1, 1994·Journal of Bioenergetics and Biomembranes·Y Wada, Y Anraku
Jan 27, 1995·Cell·D E Clapham
Oct 1, 1994·The Journal of Experimental Medicine·S N MorenoR Docampo
Jul 1, 1994·Molecular and Biochemical Parasitology·M A YakubuF Kierszenbaum
Nov 15, 1994·The Biochemical Journal·A E VercesiR Docampo
Jul 1, 1994·Physiological Reviews·T PozzanJ Meldolesi
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A D ShortD L Gill
Jan 15, 1996·The Biochemical Journal·S N Moreno, L Zhong
Apr 1, 1996·The Biochemical Journal·A E Vercesi, R Docampo

❮ Previous
Next ❯

Citations

Jun 17, 2009·The Journal of Eukaryotic Microbiology·Silvia N J Moreno, Roberto Docampo
Mar 26, 2011·PloS One·Paul N UlrichRoberto Docampo
Jun 5, 2004·Biochimica Et Biophysica Acta·Danuza Nogueira Moysés, Hector Barrabin
Feb 4, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Roberto DocampoSilvia N J Moreno
Oct 12, 2000·Anais Da Academia Brasileira De Ciências·W de SouzaN L Silva
May 24, 2003·Memórias do Instituto Oswaldo Cruz·Wanderley de Souza, Narcisa Leal da Cunha-e-Silva
Jun 10, 2000·Memórias do Instituto Oswaldo Cruz·W de Souza
Feb 19, 2017·Molecular and Biochemical Parasitology·María Carolina Pérez-GordonesMarta Mendoza
Mar 19, 2002·Cellular Microbiology·Noris M RodriguezDavid A Scott
May 10, 2001·Cell Structure and Function·A BouchotJ M Pinon
Jun 23, 2006·Parasitology Research·Adriana B FernandesRenato A Mortara
Feb 11, 2005·The Journal of Eukaryotic Microbiology·Kildare MirandaRoberto Docampo
Mar 2, 2005·Nature Reviews. Microbiology·Roberto DocampoSilvia N J Moreno
Dec 19, 2001·The Journal of Biological Chemistry·Norma MarchesiniRoberto Docampo
Nov 30, 2019·Parasite : Journal De La Société Française De Parasitologie·John C Meade
Jun 5, 2003·The Journal of Biological Chemistry·Manfredo SeufferheldRoberto Docampo
Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Malcolm J McConvilleRohan D Teasdale
May 19, 2000·The Journal of Biological Chemistry·D A Scott, R Docampo
Jun 21, 2018·Genes·Srinivasan Ramakrishnan, Roberto Docampo
Jun 21, 2019·Current Medicinal Chemistry·Alejandra Cecilia SchoijetGuillermo Daniel Alonso
Aug 15, 1998·The Journal of Biological Chemistry·D A ScottR Docampo
Sep 10, 2005·The Biochemical Journal·Mauricio VieiraRoberto Docampo
Jun 28, 2011·The Journal of Eukaryotic Microbiology·Lia Carolina Soares MedeirosKildare Miranda
Jan 10, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·Roberto Docampo, Guozhong Huang
May 10, 2000·Molecular and Biochemical Parasitology·S K GhoshJ Samuelson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.