Ca2+ dependency of 'Ca2+-independent' exocytosis in SPOC1 airway goblet cells

The Journal of Physiology
Andrea H RossiC William Davis

Abstract

SPOC1 airway goblet cells secrete mucin in response to P2Y2 receptor agonists and to secretagogues, phorbol 12-myristate 13-acetate (PMA) and ionomycin, which mobilize elements of the phospholipase C pathway, PKC and Ca2+, respectively. Previous studies demonstrated that mucin secretion from SLO-permeabilized, EGTA-buffered SPOC1 cells was stimulated by PMA at low Ca2+ levels (< 0.1 microm), consistent with the notion that regulated exocytosis may occur by Ca2+-independent pathways. We tested the alternative hypothesis that PMA-induced mucin secretion is, in fact, a Ca2+-dependent process under the conditions of low bulk Ca2+, one that is permitted in the typical SLO-permeabilized cell model by the slow binding kinetics of EGTA. Both IP3 and elevated bulk Ca2+ activated mucin secretion in SPOC1 cells buffered by EGTA, suggesting that IP3 generates a local Ca2+ gradient in the vicinity of the secretory granules to the degree necessary to trigger exocytosis. BAPTA, which binds Ca2+ approximately 100-fold faster than EGTA, diminished IP3-induced mucin release over a range of concentrations by > or = 69%, yet maintained an essentially normal mucin secretory response to elevated bulk Ca2+ in permeabilized SPOC1 cells. BAPTA also dim...Continue Reading

References

Jan 1, 1992·Methods in Enzymology·B D Gomperts, P E Tatham
May 1, 1991·British Journal of Pharmacology·K C Kim, B C Lee
Dec 18, 1981·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P F Baker, D E Knight
Jan 1, 1984·The American Journal of Physiology·D J Miller, G L Smith
Jan 1, 1995·Annual Review of Pharmacology and Toxicology·T K HardenR A Nicholas
Jun 1, 1994·The American Journal of Physiology·Y T XuanA R Whorton
Jan 28, 1993·Nature·M J Berridge
Feb 1, 1996·American Journal of Respiratory Cell and Molecular Biology·S H RandellP Nettesheim
Aug 5, 1998·The American Journal of Physiology·C E ScottC W Davis
Jun 14, 2000·Current Opinion in Neurobiology·N BroseJ Rettig
Sep 27, 2000·The Journal of General Physiology·D S KohB Hille
Nov 6, 2001·American Journal of Respiratory Cell and Molecular Biology·Y ChenR Wu
Dec 12, 2001·The Journal of Biological Chemistry·Thomas C Südhof
Jan 30, 2002·Diabetes·Stefan H Gerber, Thomas C Südhof
Mar 6, 2002·The Journal of Clinical Investigation·Michael R Knowles, Richard C Boucher
Mar 20, 2002·Cell Biochemistry and Biophysics·P J Bauer
Mar 20, 2002·Molecular Pharmacology·Marcelo G Kazanietz
Apr 5, 2002·Neuron·T F J Martin
Jul 3, 2002·Nature Reviews. Molecular Cell Biology·Edwin R Chapman
Dec 7, 2002·The International Journal of Biochemistry & Cell Biology·Duncan F Rogers
Jan 21, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jason D ConwayC William Davis
Feb 18, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Lubna H AbdullahC William Davis

❮ Previous
Next ❯

Citations

Feb 12, 2009·The Journal of Biological Chemistry·Michael J TuvimBurton F Dickey
Dec 17, 2008·Current Opinion in Pulmonary Medicine·Christopher M EvansBurton F Dickey
Nov 9, 2007·Annual Review of Physiology·C William Davis, Burton F Dickey
Jul 3, 2004·American Journal of Respiratory Cell and Molecular Biology·Philip A KempAlan D Jackson
Jun 10, 2006·American Journal of Respiratory Cell and Molecular Biology·Hays W J YoungMichael R Blackburn
Oct 30, 2010·Proceedings of the American Thoracic Society·Carla M P Ribeiro, Richard C Boucher
Jun 1, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Juan Perez-VilarRichard C Boucher
Sep 3, 2004·American Journal of Physiology. Cell Physiology·Camille EhreC William Davis
Apr 19, 2018·Reproduction, Fertility, and Development·Marta SkelinMarija Urlin
Nov 30, 2018·The Journal of Biological Chemistry·Gerard Cantero-RecasensVivek Malhotra
Nov 28, 2009·Acta Physiologica Hungarica·J I Székely, A Pataki
Sep 5, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Andrea H RossiC William Davis
Aug 31, 2007·American Journal of Physiology. Cell Physiology·Camille EhreC William Davis
Feb 9, 2008·The Journal of Physiology·Yunxiang ZhuC William Davis
Sep 4, 2015·Physiological Reviews·Jonathan H Widdicombe, Jeffrey J Wine
Apr 4, 2019·American Journal of Respiratory Cell and Molecular Biology·Aurelie PetitArnaud Bourdin
Oct 28, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Omar R FarahJaques Belik
Feb 9, 2013·Molecular and Cellular Biochemistry·Jia ZhouXiangdong Zhou
Jul 10, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Lubna H Abdullah, C William Davis
Jul 18, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jonathan H Widdicombe
Aug 1, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Diane F LeeAlison B Lansley

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

American Journal of Physiology. Lung Cellular and Molecular Physiology
Andrea H RossiC William Davis
American Journal of Respiratory Cell and Molecular Biology
Philip A KempAlan D Jackson
Annual Review of Physiology
C William Davis, Burton F Dickey
© 2022 Meta ULC. All rights reserved