PMID: 2495732Apr 1, 1989Paper

Ca2+-dependent and -independent secretagogue action on gastric mucus secretion in rabbit mucosal explants

The American Journal of Physiology
U Seidler, K F Sewing

Abstract

The secretion of high-molecular weight glycoprotein was studied in rabbit antral and fundic explants in response to acetylcholine (ACh), the calcium ionophore A23187, the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), forskolin, histamine, pentagastrin, and prostaglandin E2 (PGE2). Glycoprotein secretion was measured in pulse-chase experiments by incorporation of [14C]N-acetyl-D-glucosamine into explants and quantification of the secreted high-molecular weight-labeled glycoprotein. All tested agents except histamine and pentagastrin stimulated glycoprotein secretion in a concentration-dependent manner. The response to A23187 was abolished by removal of extracellular Ca2+ and greatly reduced by the calmodulin antagonist N-(6-amino-hexyl)-l-naphthalenesulfonamide (W7). The response to ACh was abolished by equimolar concentrations of atropine and by depletion of intracellular Ca2+ stores, an effect which was reversible on addition of Ca2+, and reduced by W7. The response to forskolin and PGE2 was not significantly affected by either extracellular or intracellular Ca2+ depletion. The combination of ACh with forskolin and A23187 with TPA had a synergistic effect on secretion. The absolute dependence of cholinergic stimula...Continue Reading

Citations

Jul 18, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·T IchikawaK Hotta
Jan 17, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·M A OstuniJ-J Lacapere
Sep 4, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Chikao ShimamotoTakashi Nakahari
Mar 20, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·H TeraokaT Ohta

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