Ca2+-dependent phosphoregulation of the plasma membrane Ca2+-ATPase ACA8 modulates stimulus-induced calcium signatures

Journal of Experimental Botany
Alex CostaMaria Cristina Bonza

Abstract

Ca2+ signals are transient, hence, upon a stimulus-induced increase in cytosolic Ca2+ concentration, cells have to re-establish resting Ca2+ levels. Ca2+ extrusion is operated by a wealth of transporters, such as Ca2+ pumps and Ca2+/H+ antiporters, which often require a rise in Ca2+ concentration to be activated. Here, we report a regulatory fine-tuning mechanism of the Arabidopsis thaliana plasma membrane-localized Ca2+-ATPase isoform ACA8 that is mediated by calcineurin B-like protein (CBL) and CBL-interacting protein kinase (CIPK) complexes. We show that two CIPKs (CIPK9 and CIPK14) are able to interact with ACA8 in vivo and phosphorylate it in vitro. Transient co-overexpression of ACA8 with CIPK9 and the plasma membrane Ca2+ sensor CBL1 in tobacco leaf cells influences nuclear Ca2+ dynamics, specifically reducing the height of the second peak of the wound-induced Ca2+ transient. Stimulus-induced Ca2+ transients in mature leaves and seedlings of an aca8 T-DNA insertion line exhibit altered dynamics when compared with the wild type. Altogether our results identify ACA8 as a prominent in vivo regulator of cellular Ca2+ dynamics and reveal the existence of a Ca2+-dependent CBL-CIPK-mediated regulatory feedback mechanism, which ...Continue Reading

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Citations

May 17, 2018·Journal of Experimental Botany·Alex CostaIldiko Szabo
Apr 28, 2018·Journal of Experimental Botany·K R KonradR Hedrich
May 3, 2018·Journal of Experimental Botany·Myriam Charpentier
Jul 17, 2018·Plant & Cell Physiology·Takashi OkamotoTaku Takahashi
Mar 5, 2020·The Biochemical Journal·Sibaji K SanyalGirdhar K Pandey
May 23, 2018·International Journal of Molecular Sciences·Kjell De VrieseSteffen Vanneste
Sep 14, 2017·Journal of Experimental Botany·Angus Murphy
Aug 14, 2020·International Journal of Molecular Sciences·Xiao MaZhen-Hui Gong
Nov 20, 2019·Journal of Experimental Botany·Julián García BossiJosé M Estevez
Jul 22, 2020·Proceedings of the National Academy of Sciences of the United States of America·Richard HillearySimon Gilroy
Jan 15, 2018·The New Phytologist·Jörg KudlaKarin Schumacher
Jan 30, 2020·Proceedings of the National Academy of Sciences of the United States of America·Pengcheng WangJian-Kang Zhu
Dec 29, 2020·Frontiers in Plant Science·Isaac Vega-MuñozSimon Stael
Mar 26, 2021·Frontiers in Plant Science·Zahra IqbalMohammad Israil Ansari
Feb 13, 2021·The New Phytologist·Julian DindasM Rob G Roelfsema
Mar 30, 2021·Plant Science : an International Journal of Experimental Plant Biology·Chanjuan LiangXiaoqian Ren
Apr 28, 2021·Plant Physiology·Francesca ResentiniAlex Costa
Nov 23, 2021·Frontiers in Bioengineering and Biotechnology·Jianmei FuBiao Liu
Nov 9, 2021·The Plant Journal : for Cell and Molecular Biology·Poonam KanwarGirdhar K Pandey

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